RRC ID 80784
Author Mullan TW, Felton T, Tam J, Kasem O, Yeung TJ, Memar N, Schnabel R, Poole RJ.
Title Control of successive unequal cell divisions by neural cell fate regulators determines embryonic neuroblast cell size.
Journal Development
Abstract Asymmetric cell divisions often generate daughter cells of unequal size in addition to different fates. In some contexts, daughter cell size asymmetry is thought to be a key input to specific binary cell fate decisions. An alternative possibility is that unequal division is a mechanism by which a variety of cells of different sizes are generated during embryonic development. We show here that two unequal cell divisions precede neuroblast formation in the C lineage of Caenorhabditis elegans. The equalisation of these divisions in a pig-1/MELK mutant background has little effect on neuroblast specification. Instead, we demonstrate that let-19/MDT13 is a regulator of the proneural basic helix-loop-helix transcription factor hlh-14/ASCL1 and find that both are required to concomitantly regulate the acquisition of neuroblast identity and neuroblast cell size. Thus, embryonic neuroblast cell size in this lineage is progressively regulated in parallel with identity by key neural cell fate regulators. We propose that key cell fate determinants have a previously unappreciated function in regulating unequal cleavage, and therefore cell size, of the progenitor cells whose daughter cell fates they then go on to specify.
Volume 151(3)
Published 2024-2-1
DOI 10.1242/dev.200981
PII 342707
PMID 38205939
PMC PMC10911278
MeSH Animals Caenorhabditis elegans Caenorhabditis elegans Proteins* / genetics Cell Division Cell Size Neural Stem Cells* Neurons
C.elegans tm295 tm1510