論文 - 詳細
| RRC ID | 80785 |
|---|---|
| 著者 | Li X, Liu B, Wen Y, Wang J, Guo YR, Shi A, Lin L. |
| タイトル | Coordination of RAB-8 and RAB-11 during unconventional protein secretion. |
| ジャーナル | J Cell Biol |
| Abstract |
Multiple physiology-pertinent transmembrane proteins reach the cell surface via the Golgi-bypassing unconventional protein secretion (UcPS) pathway. By employing C. elegans-polarized intestine epithelia, we recently have revealed that the small GTPase RAB-8/Rab8 serves as an important player in the process. Nonetheless, its function and the relevant UcPS itinerary remain poorly understood. Here, we show that deregulated RAB-8 activity resulted in impaired apical UcPS, which increased sensitivity to infection and environmental stress. We also identified the SNARE VTI-1/Vti1a/b as a new RAB-8-interacting factor involved in the apical UcPS. Besides, RAB-11/Rab11 was capable of recruiting RABI-8/Rabin8 to reduce the guanine nucleotide exchange activity of SMGL-1/GEF toward RAB-8, indicating the necessity of a finely tuned RAB-8/RAB-11 network. Populations of RAB-8- and RAB-11-positive endosomal structures containing the apical UcPS cargo moved toward the apical side. In the absence of RAB-11 or its effectors, the cargo was retained in RAB-8- and RAB-11-positive endosomes, respectively, suggesting that these endosomes are utilized as intermediate carriers for the UcPS. |
| 巻・号 | 223(2) |
| 公開日 | 2024-2-5 |
| DOI | 10.1083/jcb.202306107 |
| PII | 276436 |
| PMID | 38019180 |
| PMC | PMC10686230 |
| MeSH | Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Membrane Endosomes Protein Transport rab GTP-Binding Proteins* / metabolism |
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オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 41 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm2518 tm2526 |