| RRC ID |
80795
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| 著者 |
Medwig-Kinney TN, Kinney BA, Martinez MAQ, Yee C, Sirota SS, Mullarkey AA, Somineni N, Hippler J, Zhang W, Shen K, Hammell C, Pani AM, Matus DQ.
|
| タイトル |
Dynamic compartmentalization of the pro-invasive transcription factor NHR-67 reveals a role for Groucho in regulating a proliferative-invasive cellular switch in C. elegans.
|
| ジャーナル |
Elife
|
| Abstract |
A growing body of evidence suggests that cell division and basement membrane invasion are mutually exclusive cellular behaviors. How cells switch between proliferative and invasive states is not well understood. Here, we investigated this dichotomy in vivo by examining two cell types in the developing Caenorhabditis elegans somatic gonad that derive from equipotent progenitors, but exhibit distinct cell behaviors: the post-mitotic, invasive anchor cell and the neighboring proliferative, non-invasive ventral uterine (VU) cells. We show that the fates of these cells post-specification are more plastic than previously appreciated and that levels of NHR-67 are important for discriminating between invasive and proliferative behavior. Transcription of NHR-67 is downregulated following post-translational degradation of its direct upstream regulator, HLH-2 (E/Daughterless) in VU cells. In the nuclei of VU cells, residual NHR-67 protein is compartmentalized into discrete punctae that are dynamic over the cell cycle and exhibit liquid-like properties. By screening for proteins that colocalize with NHR-67 punctae, we identified new regulators of uterine cell fate maintenance: homologs of the transcriptional co-repressor Groucho (UNC-37 and LSY-22), as well as the TCF/LEF homolog POP-1. We propose a model in which the association of NHR-67 with the Groucho/TCF complex suppresses the default invasive state in non-invasive cells, which complements transcriptional regulation to add robustness to the proliferative-invasive cellular switch in vivo.
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| 巻・号 |
12
|
| 公開日 |
2023-12-1
|
| DOI |
10.7554/eLife.84355
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| PII |
84355
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| PMID |
38038410
|
| PMC |
PMC10691804
|
| MeSH |
Animals
Basic Helix-Loop-Helix Transcription Factors / metabolism
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins* / metabolism
Cell Differentiation
Gene Expression Regulation
Receptors, Cytoplasmic and Nuclear* / metabolism
Transcription Factors* / metabolism
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| リソース情報 |
| 線虫 |
tm4063 |
