論文 - 詳細
| RRC ID | 80808 |
|---|---|
| 著者 | Martin SCT, Qadota H, Oberhauser AF, Hardin J, Benian GM. |
| タイトル | FARL-11 (STRIP1/2) is required for sarcomere and sarcoplasmic reticulum organization in C. elegans. |
| ジャーナル | Mol Biol Cell |
| Abstract |
Protein phosphatase 2A (PP2A) functions in a variety of cellular contexts. PP2A can assemble into four different complexes based on the inclusion of different regulatory or targeting subunits. The B''' regulatory subunit "striatin" forms the STRIPAK complex consisting of striatin, a catalytic subunit (PP2AC), striatin-interacting protein 1 (STRIP1), and MOB family member 4 (MOB4). In yeast and Caenorhabditis elegans, STRIP1 is required for formation of the endoplasmic reticulum (ER). Because the sarcoplasmic reticulum (SR) is the highly organized muscle-specific version of ER, we sought to determine the function of the STRIPAK complex in muscle using C. elegans. CASH-1 (striatin) and FARL-11 (STRIP1/2) form a complex in vivo, and each protein is localized to SR. Missense mutations and single amino acid losses in farl-11 and cash-1 each result in similar sarcomere disorganization. A missense mutation in farl-11 shows no detectable FARL-11 protein by immunoblot, disruption of SR organization around M-lines, and altered levels of the SR Ca+2 release channel UNC-68. |
| 巻・号 | 34(9) |
| ページ | ar86 |
| 公開日 | 2023-8-1 |
| DOI | 10.1091/mbc.E23-03-0083 |
| PMID | 37314837 |
| PMC | PMC10398898 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Endoplasmic Reticulum / metabolism Protein Phosphatase 2 / metabolism Sarcomeres / metabolism Sarcoplasmic Reticulum* / metabolism |
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm6233 |