論文 - 詳細
| RRC ID | 80817 |
|---|---|
| 著者 | Ohnishi K, Sokabe T, Miura T, Tominaga M, Ohta A, Kuhara A. |
| タイトル | G protein-coupled receptor-based thermosensation determines temperature acclimatization of Caenorhabditis elegans. |
| ジャーナル | Nat Commun |
| Abstract |
Animals must sense and acclimatize to environmental temperatures for survival, yet their thermosensing mechanisms other than transient receptor potential (TRP) channels remain poorly understood. We identify a trimeric G protein-coupled receptor (GPCR), SRH-40, which confers thermosensitivity in sensory neurons regulating temperature acclimatization in Caenorhabditis elegans. Systematic knockdown of 1000 GPCRs by RNAi reveals GPCRs involved in temperature acclimatization, among which srh-40 is highly expressed in the ADL sensory neuron, a temperature-responsive chemosensory neuron, where TRP channels act as accessorial thermoreceptors. In vivo Ca2+ imaging demonstrates that an srh-40 mutation reduced the temperature sensitivity of ADL, resulting in supranormal temperature acclimatization. Ectopically expressing SRH-40 in a non-warmth-sensing gustatory neuron confers temperature responses. Moreover, temperature-dependent SRH-40 activation is reconstituted in Drosophila S2R+ cells. Overall, SRH-40 may be involved in thermosensory signaling underlying temperature acclimatization. We propose a dual thermosensing machinery through a GPCR and TRP channels in a single sensory neuron. |
| 巻・号 | 15(1) |
| ページ | 1660 |
| 公開日 | 2024-2-23 |
| DOI | 10.1038/s41467-024-46042-z |
| PII | 10.1038/s41467-024-46042-z |
| PMID | 38396085 |
| PMC | PMC10891075 |
| MeSH | Acclimatization / genetics Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins* / genetics Receptors, G-Protein-Coupled / genetics Sensory Receptor Cells / physiology Temperature |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 8 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| 線虫 | tm897 tm6403 |