| RRC ID |
80817
|
| Author |
Ohnishi K, Sokabe T, Miura T, Tominaga M, Ohta A, Kuhara A.
|
| Title |
G protein-coupled receptor-based thermosensation determines temperature acclimatization of Caenorhabditis elegans.
|
| Journal |
Nat Commun
|
| Abstract |
Animals must sense and acclimatize to environmental temperatures for survival, yet their thermosensing mechanisms other than transient receptor potential (TRP) channels remain poorly understood. We identify a trimeric G protein-coupled receptor (GPCR), SRH-40, which confers thermosensitivity in sensory neurons regulating temperature acclimatization in Caenorhabditis elegans. Systematic knockdown of 1000 GPCRs by RNAi reveals GPCRs involved in temperature acclimatization, among which srh-40 is highly expressed in the ADL sensory neuron, a temperature-responsive chemosensory neuron, where TRP channels act as accessorial thermoreceptors. In vivo Ca2+ imaging demonstrates that an srh-40 mutation reduced the temperature sensitivity of ADL, resulting in supranormal temperature acclimatization. Ectopically expressing SRH-40 in a non-warmth-sensing gustatory neuron confers temperature responses. Moreover, temperature-dependent SRH-40 activation is reconstituted in Drosophila S2R+ cells. Overall, SRH-40 may be involved in thermosensory signaling underlying temperature acclimatization. We propose a dual thermosensing machinery through a GPCR and TRP channels in a single sensory neuron.
|
| Volume |
15(1)
|
| Pages |
1660
|
| Published |
2024-2-23
|
| DOI |
10.1038/s41467-024-46042-z
|
| PII |
10.1038/s41467-024-46042-z
|
| PMID |
38396085
|
| PMC |
PMC10891075
|
| MeSH |
Acclimatization / genetics
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans Proteins* / genetics
Receptors, G-Protein-Coupled / genetics
Sensory Receptor Cells / physiology
Temperature
|
| Resource |
| C.elegans |
tm897
tm6403 |
