RRC ID 80817
Author Ohnishi K, Sokabe T, Miura T, Tominaga M, Ohta A, Kuhara A.
Title G protein-coupled receptor-based thermosensation determines temperature acclimatization of Caenorhabditis elegans.
Journal Nat Commun
Abstract Animals must sense and acclimatize to environmental temperatures for survival, yet their thermosensing mechanisms other than transient receptor potential (TRP) channels remain poorly understood. We identify a trimeric G protein-coupled receptor (GPCR), SRH-40, which confers thermosensitivity in sensory neurons regulating temperature acclimatization in Caenorhabditis elegans. Systematic knockdown of 1000 GPCRs by RNAi reveals GPCRs involved in temperature acclimatization, among which srh-40 is highly expressed in the ADL sensory neuron, a temperature-responsive chemosensory neuron, where TRP channels act as accessorial thermoreceptors. In vivo Ca2+ imaging demonstrates that an srh-40 mutation reduced the temperature sensitivity of ADL, resulting in supranormal temperature acclimatization. Ectopically expressing SRH-40 in a non-warmth-sensing gustatory neuron confers temperature responses. Moreover, temperature-dependent SRH-40 activation is reconstituted in Drosophila S2R+ cells. Overall, SRH-40 may be involved in thermosensory signaling underlying temperature acclimatization. We propose a dual thermosensing machinery through a GPCR and TRP channels in a single sensory neuron.
Volume 15(1)
Pages 1660
Published 2024-2-23
DOI 10.1038/s41467-024-46042-z
PII 10.1038/s41467-024-46042-z
PMID 38396085
PMC PMC10891075
MeSH Acclimatization / genetics Animals Caenorhabditis elegans* / genetics Caenorhabditis elegans Proteins* / genetics Receptors, G-Protein-Coupled / genetics Sensory Receptor Cells / physiology Temperature
Resource
C.elegans tm897 tm6403