| RRC ID |
80831
|
| Author |
Sun CL, Van Gilst M, Crowder CM.
|
| Title |
Hypoxia-induced mitochondrial stress granules.
|
| Journal |
Cell Death Dis
|
| Abstract |
Perturbations of mitochondrial proteostasis have been associated with aging, neurodegenerative diseases, and recently with hypoxic injury. While examining hypoxia-induced mitochondrial protein aggregation in C. elegans, we found that sublethal hypoxia, sodium azide, or heat shock-induced abundant ethidium bromide staining mitochondrial granules that preceded evidence of protein aggregation. Genetic manipulations that reduce cellular and organismal hypoxic death block the formation of these mitochondrial stress granules (mitoSG). Knockdown of mitochondrial nucleoid proteins also blocked the formation of mitoSG by a mechanism distinct from the mitochondrial unfolded protein response. Lack of the major mitochondrial matrix protease LONP-1 resulted in the constitutive formation of mitoSG without external stress. Ethidium bromide-staining RNA-containing mitochondrial granules were also observed in rat cardiomyocytes treated with sodium azide, a hypoxia mimetic. Mitochondrial stress granules are an early mitochondrial pathology controlled by LONP and the nucleoid, preceding hypoxia-induced protein aggregation.
|
| Volume |
14(7)
|
| Pages |
448
|
| Published |
2023-7-19
|
| DOI |
10.1038/s41419-023-05988-6
|
| PII |
10.1038/s41419-023-05988-6
|
| PMID |
37468471
|
| PMC |
PMC10356818
|
| MeSH |
Animals
Caenorhabditis elegans* / metabolism
Ethidium / metabolism
Hypoxia / metabolism
Mitochondrial Proteins / genetics
Mitochondrial Proteins / metabolism
Protein Aggregates*
Rats
Sodium Azide
Stress Granules
|
| Resource |
| C.elegans |
tm6212
tm3586
tm5171
tm4919 |
