| RRC ID |
80853
|
| Author |
Kawasaki I, Sugiura K, Sasaki T, Matsuda N, Sato M, Sato K.
|
| Title |
MARC-3, a membrane-associated ubiquitin ligase, is required for fast polyspermy block in Caenorhabditis elegans.
|
| Journal |
Nat Commun
|
| Abstract |
In many sexually reproducing organisms, oocytes are fundamentally fertilized with one sperm. In Caenorhabditis elegans, chitin layer formation after fertilization by the EGG complex is one of the mechanisms of polyspermy block, but other mechanisms remain unknown. Here, we demonstrate that MARC-3, a membrane-associated RING-CH-type ubiquitin ligase that localizes to the plasma membrane and cortical puncta in oocytes, is involved in fast polyspermy block. During polyspermy, the second sperm entry occurs within approximately 10 s after fertilization in MARC-3-deficient zygotes, whereas it occurs approximately 200 s after fertilization in egg-3 mutant zygotes defective in the chitin layer formation. MARC-3 also functions in the selective degradation of maternal plasma membrane proteins and the transient accumulation of endosomal lysine 63-linked polyubiquitin after fertilization. The RING-finger domain of MARC-3 is required for its in vitro ubiquitination activity and polyspermy block, suggesting that a ubiquitination-mediated mechanism sequentially regulates fast polyspermy block and maternal membrane protein degradation during the oocyte-to-embryo transition.
|
| Volume |
15(1)
|
| Pages |
792
|
| Published |
2024-1-26
|
| DOI |
10.1038/s41467-024-44928-6
|
| PII |
10.1038/s41467-024-44928-6
|
| PMID |
38278786
|
| PMC |
PMC10817901
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Chitin / metabolism
Fertilization / physiology
Ligases / metabolism
Male
Membrane Proteins / genetics
Membrane Proteins / metabolism
Oocytes / metabolism
Semen
Sperm-Ovum Interactions / physiology
Spermatozoa / metabolism
Ubiquitin* / metabolism
|
| Resource |
| C.elegans |
tm1626
tm3546
tm5426
tm1191 |
