論文 - 詳細
| RRC ID | 80880 |
|---|---|
| 著者 | Thomas L, Taleb Ismail B, Askjaer P, Seydoux G. |
| タイトル | Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly. |
| ジャーナル | EMBO J |
| Abstract |
Nucleoporins (Nups) assemble nuclear pores that form the permeability barrier between nucleoplasm and cytoplasm. Nucleoporins also localize in cytoplasmic foci proposed to function as pore pre-assembly intermediates. Here, we characterize the composition and incidence of cytoplasmic Nup foci in an intact animal, C. elegans. We find that, in young non-stressed animals, Nup foci only appear in developing sperm, oocytes and embryos, tissues that express high levels of nucleoporins. The foci are condensates of highly cohesive FG repeat-containing nucleoporins (FG-Nups), which are maintained near their solubility limit in the cytoplasm by posttranslational modifications and chaperone activity. Only a minor fraction of FG-Nup molecules concentrate in Nup foci, which dissolve during M phase and are dispensable for nuclear pore assembly. Nucleoporin condensation is enhanced by stress and advancing age, and overexpression of a single FG-Nup in post-mitotic neurons is sufficient to induce ectopic condensation and organismal paralysis. We speculate that Nup foci are non-essential and potentially toxic condensates whose assembly is actively suppressed in healthy cells. |
| 巻・号 | 42(13) |
| ページ | e112987 |
| 公開日 | 2023-7-3 |
| DOI | 10.15252/embj.2022112987 |
| PMID | 37254647 |
| PMC | PMC10308366 |
| MeSH | Active Transport, Cell Nucleus Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism Cell Nucleus / metabolism Male Nuclear Pore* / genetics Nuclear Pore* / metabolism Nuclear Pore Complex Proteins* / genetics Nuclear Pore Complex Proteins* / metabolism Semen / metabolism |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 13 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.2 |
| リソース情報 | |
| 線虫 | tm3039 |