Reference - Detail
| RRC ID | 80891 |
|---|---|
| Author | Pastore B, Hertz HL, Tang W. |
| Title | Pre-piRNA trimming safeguards piRNAs against erroneous targeting by RNA-dependent RNA polymerase. |
| Journal | Cell Rep |
| Abstract |
The Piwi/Piwi-interacting RNA (piRNA) pathway protects genome integrity in animal germ lines. Maturation of piRNAs involves nucleolytic processing at both 5' and 3' ends. The ribonuclease PARN-1 and its orthologs mediate piRNA 3' trimming in worms, insects, and mammals. However, the significance of this evolutionarily conserved processing step is not fully understood. Employing C. elegans as a model, we recently discovered that 3' trimming protects piRNAs against non-templated nucleotide additions and degradation. Here, we find that worms lacking PARN-1 accumulate an uncharacterized RNA species termed anti-piRNAs, which are antisense to piRNAs. Anti-piRNAs associate with Piwi proteins, are 17-19 nucleotides long, and begin with 5' guanine or adenine. Untrimmed pre-piRNAs are misdirected by the terminal nucleotidyltransferase RDE-3 and RNA-dependent RNA polymerase EGO-1, leading to the formation of anti-piRNAs. This work identifies a class of small RNAs in parn-1 mutants and provides insight into the activities of RDE-3, EGO-1, and Piwi proteins. |
| Volume | 43(2) |
| Pages | 113692 |
| Published | 2024-2-27 |
| DOI | 10.1016/j.celrep.2024.113692 |
| PII | S2211-1247(24)00020-2 |
| PMID | 38244197 |
| PMC | PMC10949418 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins* / genetics Mammals Nucleotides Nucleotidyltransferases Piwi-Interacting RNA RNA RNA-Dependent RNA Polymerase* Ribonucleases |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm869 tm872 |