論文 - 詳細
| RRC ID | 80907 |
|---|---|
| 著者 | Okahata M, Sawada N, Nakao K, Ohta A, Kuhara A. |
| タイトル | Screening for cold tolerance genes in C. elegans, whose expressions are affected by anticancer drugs camptothecin and leptomycin B. |
| ジャーナル | Sci Rep |
| Abstract |
Temperature is a vital environmental factor affecting organisms' survival as they determine the mechanisms to tolerate rapid temperature changes. We demonstrate an experimental system for screening chemicals that affect cold tolerance in Caenorhabditis elegans. The anticancer drugs leptomycin B and camptothecin were among the 4000 chemicals that were screened as those affecting cold tolerance. Genes whose expression was affected by leptomycin B or camptothecin under cold stimuli were investigated by transcriptome analysis. Abnormal cold tolerance was detected in several mutants possessing genes that were rendered defective and whose expression altered after exposure to either leptomycin B or camptothecin. The genetic epistasis analysis revealed that leptomycin B or camptothecin may increase cold tolerance by affecting a pathway upstream of the insulin receptor DAF-2 that regulates cold tolerance in the intestine. Our experimental system combining drug and cold tolerance could be used for a comprehensive screening of genes that control cold tolerance at a low cost and in a short time period. |
| 巻・号 | 14(1) |
| ページ | 5401 |
| 公開日 | 2024-3-5 |
| DOI | 10.1038/s41598-024-55794-z |
| PII | 10.1038/s41598-024-55794-z |
| PMID | 38443452 |
| PMC | PMC10914781 |
| MeSH | Animals Antineoplastic Agents* Caenorhabditis elegans / genetics Camptothecin* / pharmacology Fatty Acids, Unsaturated |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm830 tm1503 tm2319 tm1711 |