論文 - 詳細
| RRC ID | 80914 |
|---|---|
| 著者 | Blundon JM, Cesar BI, Bae JW, Čavka I, Haversat J, Ries J, Köhler S, Kim Y. |
| タイトル | Skp1 proteins are structural components of the synaptonemal complex in C. elegans. |
| ジャーナル | Sci Adv |
| Abstract |
The synaptonemal complex (SC) is a zipper-like protein assembly that links homologous chromosomes to regulate recombination and segregation during meiosis. The SC has been notoriously refractory to in vitro reconstitution, thus leaving its molecular organization largely unknown. Here, we report a moonlighting function of two paralogous S-phase kinase-associated protein 1 (Skp1)-related proteins (SKR-1 and SKR-2), well-known adaptors of the Skp1-Cul1-F-box (SCF) ubiquitin ligase, as the key missing components of the SC in Caenorhabditis elegans. SKR proteins repurpose their SCF-forming interfaces to dimerize and interact with meiosis-specific SC proteins, thereby driving synapsis independent of SCF activity. SKR-1 enables the formation of the long-sought-after soluble complex with previously identified SC proteins in vitro, which we propose it to represent a complete SC building block. Our findings demonstrate how a conserved cell cycle regulator has been co-opted to interact with rapidly evolving meiotic proteins to construct the SC and provide a foundation for understanding its structure and assembly mechanisms. |
| 巻・号 | 10(7) |
| ページ | eadl4876 |
| 公開日 | 2024-2-16 |
| DOI | 10.1126/sciadv.adl4876 |
| PMID | 38354250 |
| PMC | PMC10866564 |
| MeSH | Animals Caenorhabditis elegans* / metabolism Caenorhabditis elegans Proteins* / genetics Caenorhabditis elegans Proteins* / metabolism Cell Cycle Proteins / metabolism S-Phase Kinase-Associated Proteins / genetics S-Phase Kinase-Associated Proteins / metabolism Synaptonemal Complex / metabolism |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
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| リソース情報 | |
| 線虫 | tm3655 |