| RRC ID |
80938
|
| Author |
Alexander KD, Ramachandran S, Biswas K, Lambert CM, Russell J, Oliver DB, Armstrong W, Rettler M, Liu S, Doitsidou M, Bénard C, Walker AK, Francis MM.
|
| Title |
The homeodomain transcriptional regulator DVE-1 directs a program for synapse elimination during circuit remodeling.
|
| Journal |
Nat Commun
|
| Abstract |
The elimination of synapses during circuit remodeling is critical for brain maturation; however, the molecular mechanisms directing synapse elimination and its timing remain elusive. We show that the transcriptional regulator DVE-1, which shares homology with special AT-rich sequence-binding (SATB) family members previously implicated in human neurodevelopmental disorders, directs the elimination of juvenile synaptic inputs onto remodeling C. elegans GABAergic neurons. Juvenile acetylcholine receptor clusters and apposing presynaptic sites are eliminated during the maturation of wild-type GABAergic neurons but persist into adulthood in dve-1 mutants, producing heightened motor connectivity. DVE-1 localization to GABAergic nuclei is required for synapse elimination, consistent with DVE-1 regulation of transcription. Pathway analysis of putative DVE-1 target genes, proteasome inhibitor, and genetic experiments implicate the ubiquitin-proteasome system in synapse elimination. Together, our findings define a previously unappreciated role for a SATB family member in directing synapse elimination during circuit remodeling, likely through transcriptional regulation of protein degradation processes.
|
| Volume |
14(1)
|
| Pages |
7520
|
| Published |
2023-11-18
|
| DOI |
10.1038/s41467-023-43281-4
|
| PII |
10.1038/s41467-023-43281-4
|
| PMID |
37980357
|
| PMC |
PMC10657367
|
| MeSH |
Animals
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
GABAergic Neurons / metabolism
Humans
Receptors, Cholinergic / metabolism
Synapses / metabolism
|
| Resource |
| C.elegans |
tm1597
tm495
tm4803
tm4807 |
