RRC ID 80967
Author Ikei M, Miyazaki R, Monden K, Naito Y, Takeuchi A, Takahashi YS, Tanaka Y, Murata K, Mori T, Ichikawa M, Tsukazaki T.
Title YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition.
Journal PLoS Biol
Abstract Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in Escherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via Spirochaeta thermophila YeeE in vitro and characterized E. coli YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that S. thermophila YeeD possesses thiosulfate decomposition activity and that a conserved cysteine in YeeD was modified to several forms in the presence of thiosulfate. Finally, the crystal structures of S. thermophila YeeE-YeeD fusion proteins at 3.34-Å and 2.60-Å resolutions revealed their interactions. The association was evaluated by a binding assay using purified S. thermophila YeeE and YeeD. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed.
Volume 22(4)
Pages e3002601
Published 2024-4-1
DOI 10.1371/journal.pbio.3002601
PII PBIOLOGY-D-24-00286
PMID 38656967
PMC PMC11073785
MeSH Amino Acid Sequence Bacterial Proteins / genetics Bacterial Proteins / metabolism Biological Transport / genetics Crystallography, X-Ray Cysteine / metabolism Escherichia coli* / genetics Escherichia coli* / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Protein Binding Sulfurtransferases* / genetics Sulfurtransferases* / metabolism Thiosulfates* / metabolism
Resource
DNA material Genomic DNA of Escherichia coli JCM 20135 (JGD07547)