論文 - 詳細
| RRC ID | 80967 |
|---|---|
| 著者 | Ikei M, Miyazaki R, Monden K, Naito Y, Takeuchi A, Takahashi YS, Tanaka Y, Murata K, Mori T, Ichikawa M, Tsukazaki T. |
| タイトル | YeeD is an essential partner for YeeE-mediated thiosulfate uptake in bacteria and regulates thiosulfate ion decomposition. |
| ジャーナル | PLoS Biol |
| Abstract |
Uptake of thiosulfate ions as an inorganic sulfur source from the environment is important for bacterial sulfur assimilation. Recently, a selective thiosulfate uptake pathway involving a membrane protein YeeE (TsuA) in Escherichia coli was characterized. YeeE-like proteins are conserved in some bacteria, archaea, and eukaryotes. However, the precise function of YeeE, along with its potential partner protein in the thiosulfate ion uptake pathway, remained unclear. Here, we assessed selective thiosulfate transport via Spirochaeta thermophila YeeE in vitro and characterized E. coli YeeD (TsuB) as an adjacent and essential protein for YeeE-mediated thiosulfate uptake in vivo. We further showed that S. thermophila YeeD possesses thiosulfate decomposition activity and that a conserved cysteine in YeeD was modified to several forms in the presence of thiosulfate. Finally, the crystal structures of S. thermophila YeeE-YeeD fusion proteins at 3.34-Å and 2.60-Å resolutions revealed their interactions. The association was evaluated by a binding assay using purified S. thermophila YeeE and YeeD. Based on these results, a model of the sophisticated uptake of thiosulfate ions by YeeE and YeeD is proposed. |
| 巻・号 | 22(4) |
| ページ | e3002601 |
| 公開日 | 2024-4-1 |
| DOI | 10.1371/journal.pbio.3002601 |
| PII | PBIOLOGY-D-24-00286 |
| PMID | 38656967 |
| PMC | PMC11073785 |
| MeSH | Amino Acid Sequence Bacterial Proteins / genetics Bacterial Proteins / metabolism Biological Transport / genetics Crystallography, X-Ray Cysteine / metabolism Escherichia coli* / genetics Escherichia coli* / metabolism Escherichia coli Proteins / genetics Escherichia coli Proteins / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism Protein Binding Sulfurtransferases* / genetics Sulfurtransferases* / metabolism Thiosulfates* / metabolism |
| IF | 7.076 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 35 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | Genomic DNA of Escherichia coli JCM 20135 (JGD07547) |