論文 - 詳細
| RRC ID | 80968 |
|---|---|
| 著者 | Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA. |
| タイトル | descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. |
| ジャーナル | Nat Commun |
| Abstract |
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced ( https://github.com/dbsb-juntendo/descSPIM ), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies. |
| 巻・号 | 15(1) |
| ページ | 4941 |
| 公開日 | 2024-6-12 |
| DOI | 10.1038/s41467-024-49131-1 |
| PII | 10.1038/s41467-024-49131-1 |
| PMID | 38866781 |
| PMC | PMC11169475 |
| MeSH | Animals Brain* / diagnostic imaging Cell Line, Tumor Humans Imaging, Three-Dimensional* / methods Mice Microscopy, Fluorescence* / instrumentation Microscopy, Fluorescence* / methods |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 108 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 1.0 |
| リソース情報 | |
| 実験動物マウス | RBRC06571 |
| 遺伝子材料 | pcDNA3 Venus-Akaluc (RDB15781) |