論文 - 詳細
| RRC ID | 81051 |
|---|---|
| 著者 | Mizuki Fukuda, Manato Kinkawa, Masahiro Hayashi |
| タイトル | Fed-batch cultivation of Euglena gracilis for the high-yield production and GPC-assisted molecular weight determination of paramylon |
| ジャーナル | Bioscience, Biotechnology, and Biochemistry |
| Abstract |
ABSTRACT
Paramylon, a starch-like carbohydrate accumulated in Euglena gracilis cells, could be a potential source as a raw material for chemical products; its high-yield production would thus be highly desired. Although the molecular weight and polymerization degree of paramylon are important properties for its use as a raw material for chemical products, the available information about paramylon molecular weight remains insufficient. Therefore, in this study, we investigated a high-density E. gracilis culture approach and how culture conditions affect paramylon molecular weight. The nitrogen source, cultivation temperature, and nutrient feeding were optimized for maximum biomass and paramylon productivity. The maximum dry cell weight and paramylon content yields reached 108.9 g/L and 87.2%, respectively. Paramylon molecular weight was in the range of 220 000–320 000 Da. Our gel permeation chromatography analysis showed that the cells with a higher paramylon content tended to contain paramylon of a higher molecular weight. |
| 巻・号 | 88 |
| ページ | 206-211 |
| 公開日 | 2024-1-24 |
| DOI | 10.1093/bbb/zbad152 |
| PMID | 37974047 |
| MeSH | Euglena gracilis* Glucans Molecular Weight Starch |
| IF | 1.516 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 藻類 | NIES-48 |