| RRC ID |
81052
|
| 著者 |
Zhang SS, Xiong J, Cui JJ, Ma KL, Wu WL, Li Y, Luo S, Gao K, Dong SH.
|
| タイトル |
Lanthipeptides from the Same Core Sequence: Characterization of a Class II Lanthipeptide Synthetase from Microcystis aeruginosa NIES-88.
|
| ジャーナル |
Org Lett
|
| Abstract |
Class II lanthipeptide synthetases (LanMs) are relatively promiscuous to core peptide variations. Previous studies have shown that different LanMs catalyze identical reactions on the same core sequence fused to their respective cognate leaders. We characterized a new LanM enzyme from Microcystis aeruginosa NIES-88, MalM, and demonstrated that MalM and ProcM exhibited disparate dehydration and cyclization patterns on identical core peptides. Our study provided new insights into the regioselectivity of LanMs and showcased an appropriate strategy for lanthipeptide structural diversity engineering.
|
| 巻・号 |
24(11)
|
| ページ |
2226-2231
|
| 公開日 |
2022-3-25
|
| DOI |
10.1021/acs.orglett.2c00573
|
| PMID |
35293207
|
| MeSH |
Cyclization
Ligases* / chemistry
Microcystis* / metabolism
Peptides / chemistry
Substrate Specificity
|
| IF |
6.091
|
| リソース情報 |
| 藻類 |
NIES-88 |
