RRC ID 81095
Author Mishina K, Suzuki T, Oono Y, Yamashita Y, Zhu H, Ogawa T, Ohta M, Doman K, Xu W, Takahashi D, Miyazaki T, Tagiri A, Soma C, Horita H, Nasuda S, De Oliveira R, Paux E, Chen G, Pourkheirandish M, Wu J, Liu C, Komatsuda T.
Title Wheat Ym2 originated from Aegilops sharonensis and confers resistance to soil-borne Wheat yellow mosaic virus infection to the roots.
Journal Proc Natl Acad Sci U S A
Abstract Wheat yellow mosaic virus (WYMV) is a pathogen transmitted into its host's roots by the soil-borne vector Polymyxa graminis. Ym1 and Ym2 genes protect the host from the significant yield losses caused by the virus, but the mechanistic basis of these resistance genes remains poorly understood. Here, it has been shown that Ym1 and Ym2 act within the root either by hindering the initial movement of WYMV from the vector into the root and/or by suppressing viral multiplication. A mechanical inoculation experiment on the leaf revealed that the presence of Ym1 reduced viral infection incidence, rather than viral titer, while that of Ym2 was ineffective in the leaf. To understand the basis of the root specificity of the Ym2 product, the gene was isolated from bread wheat using a positional cloning approach. The candidate gene encodes a CC-NBS-LRR protein and it correlated allelic variation with respect to its sequence with the host's disease response. Ym2 (B37500) and its paralog (B35800) are found in the near-relatives, respectively, Aegilops sharonensis and Aegilops speltoides (a close relative of the donor of bread wheat's B genome), while both sequences, in a concatenated state, are present in several accessions of the latter species. Structural diversity in Ym2 has been generated via translocation and recombination between the two genes and enhanced by the formation of a chimeric gene resulting from an intralocus recombination event. The analysis has revealed how the Ym2 region has evolved during the polyploidization events leading to the creation of cultivated wheat.
Volume 120(11)
Pages e2214968120
Published 2023-3-14
DOI 10.1073/pnas.2214968120
PMID 36897977
PMC PMC10089197
MeSH Aegilops* / genetics Aegilops* / metabolism Cloning, Molecular Phylogeny Plant Diseases Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / genetics Plant Roots / metabolism Plant Roots / virology Transcription, Genetic Triticum* / genetics Triticum* / metabolism Triticum* / virology
IF 9.412
Resource
Wheat LPGKU2329 LPGKU2348 LPGKU2343 LPGKU2327 KT020-612 LPGKU2326 LPGKU2340 KU-1215 KT020-124 LPGKU2324 LPGKU2325 LPGKU2321 KT020-029 LPGKU2323 KT020-158 LPGKU2339 LPGKU2320 KU-3136 KT020-157 LPGKU2335 LPGKU2305 LPGKU2322 LPGKU2337 LPGKU2338 KT020-617 KT020-159