Reference - Detail
| RRC ID | 81131 |
|---|---|
| Author | Naka M, Umeno T, Shibuya M, Yamaberi Y, Ueda A, Tanaka M, Takemoto H, Oba M. |
| Title | Intracellular Delivery of Plasmid DNA Using Amphipathic Helical Cell-Penetrating Peptides Containing Dipropylglycine. |
| Journal | Chem Pharm Bull (Tokyo) |
| Abstract |
Cell-penetrating peptides (CPPs) serve as potent vehicles for delivering membrane-impermeable compounds, including nucleic acids, into cells. In a previous study, we reported the successful intracellular delivery of small interfering RNAs (siRNAs) with negligible cytotoxicity using a peptide containing an unnatural amino acid (dipropylglycine). In the present study, we employed the same seven peptides as the previous study to evaluate their efficacy in delivering plasmid DNA (pDNA) intracellularly. Although pDNA and siRNA are nucleic acids, they differ in size and biological function, which may influence the optimal peptide sequences for their delivery. Herein, three peptides demonstrated effective pDNA transfection abilities. Notably, only one of the three peptides previously exhibited efficient gene-silencing effect with siRNA. These findings validate our hypothesis and offer insights for the personalized design of CPPs for the delivery of pDNA and siRNA. |
| Volume | 72(5) |
| Pages | 512-517 |
| Published | 2024-1-1 |
| DOI | 10.1248/cpb.c24-00221 |
| PMID | 38811213 |
| MeSH | Cell Survival / drug effects Cell-Penetrating Peptides* / chemistry DNA* / chemistry Glycine / chemistry HeLa Cells Humans Plasmids* RNA, Small Interfering* / administration & dosage RNA, Small Interfering* / chemistry Transfection |
| IF | 1.416 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pCAcc-Luc+ (RDB02443) |