論文 - 詳細
| RRC ID | 81233 |
|---|---|
| 著者 | Schwarz C, Johnson A, Kõivomägi M, Zatulovskiy E, Kravitz CJ, Doncic A, Skotheim JM. |
| タイトル | A Precise Cdk Activity Threshold Determines Passage through the Restriction Point. |
| ジャーナル | Mol Cell |
| Abstract |
At the restriction point (R), mammalian cells irreversibly commit to divide. R has been viewed as a point in G1 that is passed when growth factor signaling initiates a positive feedback loop of Cdk activity. However, recent studies have cast doubt on this model by claiming R occurs prior to positive feedback activation in G1 or even before completion of the previous cell cycle. Here we reconcile these results and show that whereas many commonly used cell lines do not exhibit a G1 R, primary fibroblasts have a G1 R that is defined by a precise Cdk activity threshold and the activation of cell-cycle-dependent transcription. A simple threshold model, based solely on Cdk activity, predicted with more than 95% accuracy whether individual cells had passed R. That a single measurement accurately predicted cell fate shows that the state of complex regulatory networks can be assessed using a few critical protein activities. |
| 巻・号 | 69(2) |
| ページ | 253-264.e5 |
| 公開日 | 2018-1-18 |
| DOI | 10.1016/j.molcel.2017.12.017 |
| PII | S1097-2765(17)30969-3 |
| PMID | 29351845 |
| PMC | PMC5790185 |
| MeSH | Animals Cell Cycle / physiology Cell Cycle Checkpoints / physiology* Cell Cycle Proteins / metabolism Cell Division Cell Line Cyclin-Dependent Kinase 2 / metabolism* Cyclin-Dependent Kinase 2 / physiology Cyclin-Dependent Kinases / metabolism Fibroblasts / physiology G1 Phase / physiology G1 Phase Cell Cycle Checkpoints / physiology* Humans Phosphorylation Primary Cell Culture Signal Transduction |
| IF | 15.584 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | CSII-EF-MCS (RDB04378) |