Reference - Detail
| RRC ID | 81246 |
|---|---|
| Author | Geu-Flores F, Møldrup ME, Böttcher C, Olsen CE, Scheel D, Halkier BA. |
| Title | Cytosolic γ-glutamyl peptidases process glutathione conjugates in the biosynthesis of glucosinolates and camalexin in Arabidopsis. |
| Journal | Plant Cell |
| Abstract |
The defense-related plant metabolites known as glucosinolates play important roles in agriculture, ecology, and human health. Despite an advanced biochemical understanding of the glucosinolate pathway, the source of the reduced sulfur atom in the core glucosinolate structure remains unknown. Recent evidence has pointed toward GSH, which would require further involvement of a GSH conjugate processing enzyme. In this article, we show that an Arabidopsis thaliana mutant impaired in the production of the γ-glutamyl peptidases GGP1 and GGP3 has altered glucosinolate levels and accumulates up to 10 related GSH conjugates. We also show that the double mutant is impaired in the production of camalexin and accumulates high amounts of the camalexin intermediate GS-IAN upon induction. In addition, we demonstrate that the cellular and subcellular localization of GGP1 and GGP3 matches that of known glucosinolate and camalexin enzymes. Finally, we show that the purified recombinant GGPs can metabolize at least nine of the 10 glucosinolate-related GSH conjugates as well as GS-IAN. Our results demonstrate that GSH is the sulfur donor in the biosynthesis of glucosinolates and establish an in vivo function for the only known cytosolic plant γ-glutamyl peptidases, namely, the processing of GSH conjugates in the glucosinolate and camalexin pathways. |
| Volume | 23(6) |
| Pages | 2456-69 |
| Published | 2011-6-1 |
| DOI | 10.1105/tpc.111.083998 |
| PII | tpc.111.083998 |
| PMID | 21712415 |
| PMC | PMC3160024 |
| MeSH | Animals Arabidopsis / enzymology* Arabidopsis / genetics Arabidopsis / metabolism* Arabidopsis Proteins / genetics Arabidopsis Proteins / metabolism* Cytosol / enzymology* Glucosinolates / biosynthesis* Glutathione / chemistry Glutathione / metabolism* Humans Indoles / metabolism* Mass Spectrometry Molecular Sequence Data Molecular Structure Peptide Hydrolases / genetics Peptide Hydrolases / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Sulfur / metabolism Thiazoles / metabolism* |
| IF | 9.618 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda08234 |