| RRC ID |
81258
|
| Author |
Mayuko Maeda, Miou Abe, Keisuke Aoshima, Atsushi Kobayashi, Hideto Fukushi, Takashi Kimura
|
| Title |
Identification of the Promoter Antisense Transcript Enhancing the Transcription of the Equine Herpesvirus-1 Immediate-Early Gene
|
| Journal |
Viruses
|
| Abstract |
Equine herpesvirus-1 (EHV-1) causes respiratory diseases, abortion, and encephalomyelitis in horses. The EHV-1 immediate-early (IE) protein, essential for viral replication, is transactivated by the binding of a multiprotein complex including the open reading frame 12 (ORF12) and some host factors to the IE promoter region. Promoter-associated non-coding RNAs (pancRNAs), which are transcribed from bidirectional promoters, regulate the transcription of neighboring genes in mammals and pathogens. In this study, we identified a novel pancRNA transcribed from across the areas of the 5′-untranslated region and a promoter of EHV-1 IE and named it IE pancRNA. IE pancRNA and mRNA were simultaneously expressed in EHV-1-infected RN33B-A68B2M cells. This pancRNA was also transcribed in RK13 and E. Derm cells, which are highly susceptible to EHV-1 infection. Furthermore, IE pancRNA upregulated IE gene expression in the presence of ORF12, and stable expression of IE pancRNA increased the number of EHV-1-infected RN33B-A68B2M cells. These results suggest that IE pancRNAs facilitate EHV-1 proliferation by promoting IE gene expression.
|
| Volume |
16
|
| Pages |
1195
|
| Published |
2024-7-25
|
| DOI |
10.3390/v16081195
|
| PMID |
39205169
|
| PMC |
PMC11360796
|
| MeSH |
Animals
Cell Line
Gene Expression Regulation, Viral*
Genes, Immediate-Early*
Herpesviridae Infections / veterinary
Herpesviridae Infections / virology
Herpesvirus 1, Equid* / genetics
Horse Diseases / virology
Horses
Open Reading Frames
Promoter Regions, Genetic*
RNA, Antisense / genetics
Transcription, Genetic
Virus Replication*
|
| IF |
3.816
|
| Resource |
| DNA material |
CSII-CMV-MCS-IRES2-Bsd (RDB04385)
pCMV-VSV-G-RSV-Rev (RDB04393)
pCAG-HIVgp (RDB04394) |
