| RRC ID |
81286
|
| Author |
Hernández-Díaz N, Tzouganatou S, Mulik PR, Balestrini PA, Fogarty NME.
|
| Title |
Transcriptional insights from the human embryo identify laminin-511 as a suitable matrix for human trophoblast stem cell culture.
|
| Journal |
Placenta
|
| Abstract |
The establishment of culture conditions to propagate self-renewing human trophoblast stem cells in long-term culture provides a paradigm for in vitro modelling of trophoblast. The extracellular matrix (ECM) is a critical determinant of cell identity and behaviour. Therefore, models aiming to reproduce cells in vitro should recapitulate the native cell-ECM microenvironment. Here, we mine human embryo transcriptional datasets to identify ECM components and cognate receptors expressed in the trophectoderm. Following, we identify laminin-511-E8 protein fragment as a physiologically relevant ECM capable of maintaining hTSCs in the stem cell state and retaining differentiation ability.
|
| Published |
2024-7-26
|
| DOI |
10.1016/j.placenta.2024.07.308
|
| PII |
S0143-4004(24)00602-7
|
| PMID |
39095275
|
| IF |
3.177
|
| Resource |
| Human and Animal Cells |
CT27(RCB4936) |
