Reference - Detail
| RRC ID | 81286 |
|---|---|
| Author | Hernández-Díaz N, Tzouganatou S, Mulik PR, Balestrini PA, Fogarty NME. |
| Title | Transcriptional insights from the human embryo identify laminin-511 as a suitable matrix for human trophoblast stem cell culture. |
| Journal | Placenta |
| Abstract |
The establishment of culture conditions to propagate self-renewing human trophoblast stem cells in long-term culture provides a paradigm for in vitro modelling of trophoblast. The extracellular matrix (ECM) is a critical determinant of cell identity and behaviour. Therefore, models aiming to reproduce cells in vitro should recapitulate the native cell-ECM microenvironment. Here, we mine human embryo transcriptional datasets to identify ECM components and cognate receptors expressed in the trophectoderm. Following, we identify laminin-511-E8 protein fragment as a physiologically relevant ECM capable of maintaining hTSCs in the stem cell state and retaining differentiation ability. |
| Published | 2024-7-26 |
| DOI | 10.1016/j.placenta.2024.07.308 |
| PII | S0143-4004(24)00602-7 |
| PMID | 39095275 |
| IF | 3.177 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | CT27(RCB4936) |