| RRC ID |
81322
|
| 著者 |
Suzuki M, Yasue N, Ueno N.
|
| タイトル |
Differential cellular stiffness across tissues that contribute to Xenopus neural tube closure.
|
| ジャーナル |
Dev Growth Differ
|
| Abstract |
During the formation of the neural tube, the primordium of the vertebrate central nervous system, the actomyosin activity of cells in different regions drives neural plate bending. However, how the stiffness of the neural plate and surrounding tissues is regulated and mechanically influences neural plate bending has not been elucidated. Here, we used atomic force microscopy to reveal the relationship between the stiffness of the neural plate and the mesoderm during Xenopus neural tube formation. Measurements with intact embryos revealed that the stiffness of the neural plate was consistently higher compared with the non-neural ectoderm and that it increased in an actomyosin activity-dependent manner during neural plate bending. Interestingly, measurements of isolated tissue explants also revealed that the relationship between the stiffness of the apical and basal sides of the neural plate was reversed during bending and that the stiffness of the mesoderm was lower than that of the basal side of the neural plate. The experimental elevation of mesoderm stiffness delayed neural plate bending, suggesting that low mesoderm stiffness mechanically supports neural tube closure. This study provides an example of mechanical interactions between tissues during large-scale morphogenetic movements.
|
| 巻・号 |
66(5)
|
| ページ |
320-328
|
| 公開日 |
2024-6-1
|
| DOI |
10.1111/dgd.12936
|
| PMID |
38925637
|
| PMC |
PMC11457508
|
| MeSH |
Animals
Ectoderm / cytology
Ectoderm / metabolism
Embryo, Nonmammalian / cytology
Embryo, Nonmammalian / embryology
Embryo, Nonmammalian / metabolism
Mesoderm / cytology
Mesoderm / embryology
Mesoderm / metabolism
Microscopy, Atomic Force
Neural Plate* / cytology
Neural Plate* / embryology
Neural Plate* / metabolism
Neural Tube* / cytology
Neural Tube* / embryology
Neural Tube* / metabolism
Xenopus laevis* / embryology
|
| IF |
1.723
|
| リソース情報 |
| ツメガエル・イモリ |
Xenopus genomic database
Technical assistance |
