RRC ID 81343
Author Risa Suzuki, Jia Zheng Woo, Thomas Thumberger, Gero Hofmann, Joachim Wittbrodt, Tinatini Tavhelidse-Suck
Title Characterizing medaka visual features using a high-throughput optomotor response assay
Journal PLOS ONE
Abstract Medaka fish (Oryzias latipes) is a powerful model to study genetics underlying the developmental and functional traits of the vertebrate visual system. We established a simple and high-throughput optomotor response (OMR) assay utilizing medaka larvae to study visual functions including visual acuity and contrast sensitivity. Our assay presents multiple adjustable stripes in motion to individual fish in a linear arena. For that the OMR assay employs a tablet display and the Fish Stripes software to adjust speed, width, color, and contrast of the stripes. Our results demonstrated that optomotor responses were robustly induced by black and white stripes presented from below in the linear-pool-arena. We detected robust strain specific differences in the OMR when comparing long established medaka inbred strains. We observed an interesting training effect upon the initial exposure of larvae to thick stripes, which allowed them to better respond to narrower stripes. The OMR setup and protocol presented here provide an efficient tool for quantitative phenotype mapping, addressing visual acuity, trainability of cortical neurons, color sensitivity, locomotor response, retinal regeneration and others. Our open-source setup presented here provides a crucial prerequisite for ultimately addressing the genetic basis of those processes.
Volume 19
Pages e0302092
Published 2024-6-28
DOI 10.1371/journal.pone.0302092
PMID 38941325
PMC PMC11213317
MeSH Animals Contrast Sensitivity / physiology High-Throughput Screening Assays / methods Larva* / physiology Oryzias* / physiology Photic Stimulation Vision, Ocular / physiology Visual Acuity / physiology
Resource
Medaka OK-Cab (MT830) HdrR-II1 (IB178)