| RRC ID |
81368
|
| Author |
Tanaka T, Yano T, Usuki S, Seo Y, Mizuta K, Okaguchi M, Yamaguchi M, Hanyu-Nakamura K, Toyama-Sorimachi N, Brückner K, Nakamura A.
|
| Title |
Endocytosed dsRNAs induce lysosomal membrane permeabilization that allows cytosolic dsRNA translocation for Drosophila RNAi responses.
|
| Journal |
Nat Commun
|
| Abstract |
RNA interference (RNAi) is a gene-silencing mechanism triggered by the cytosolic entry of double-stranded RNAs (dsRNAs). Many animal cells internalize extracellular dsRNAs via endocytosis for RNAi induction. However, it is not clear how the endocytosed dsRNAs are translocated into the cytosol across the endo/lysosomal membrane. Herein, we show that in Drosophila S2 cells, endocytosed dsRNAs induce lysosomal membrane permeabilization (LMP) that allows cytosolic dsRNA translocation. LMP mediated by dsRNAs requires the lysosomal Cl-/H+ antiporter ClC-b/DmOstm1. In clc-b or dmostm1 knockout S2 cells, extracellular dsRNAs are endocytosed and reach the lysosomes normally but fail to enter the cytosol. Pharmacological induction of LMP restores extracellular dsRNA-directed RNAi in clc-b or dmostm1-knockout cells. Furthermore, clc-b or dmostm1 mutant flies are defective in extracellular dsRNA-directed RNAi and its associated antiviral immunity. Therefore, endocytosed dsRNAs have an intrinsic ability to induce ClC-b/DmOstm1-dependent LMP that allows cytosolic dsRNA translocation for RNAi responses in Drosophila cells.
|
| Volume |
15(1)
|
| Pages |
6993
|
| Published |
2024-8-14
|
| DOI |
10.1038/s41467-024-51343-4
|
| PII |
10.1038/s41467-024-51343-4
|
| PMID |
39143098
|
| PMC |
PMC11324899
|
| MeSH |
Animals
Cell Line
Chloride Channels / genetics
Chloride Channels / metabolism
Cytosol* / metabolism
Drosophila / genetics
Drosophila / metabolism
Drosophila Proteins* / genetics
Drosophila Proteins* / metabolism
Drosophila melanogaster / genetics
Drosophila melanogaster / metabolism
Endocytosis*
Intracellular Membranes / metabolism
Lysosomes* / metabolism
Permeability
RNA Interference*
RNA, Double-Stranded* / metabolism
|
| Resource |
| Drosophila |
DGRC#119050
DGRC#119051
DGRC#119052
DGRC#119053 |
