論文 - 詳細
| RRC ID | 81381 |
|---|---|
| 著者 | Kitagawa T, Kobayashi D, Baron B, Okita H, Miyamoto T, Takai R, Paudel D, Ohta T, Asaoka Y, Tokunaga M, Nakagawa K, Furutani-Seiki M, Araki N, Kuramitsu Y, Kobayashi M. |
| タイトル | AT-hook DNA-binding motif-containing protein one knockdown downregulates EWS-FLI1 transcriptional activity in Ewing's sarcoma cells. |
| ジャーナル | PLoS One |
| Abstract |
Ewing's sarcoma is the second most common bone malignancy in children or young adults and is caused by an oncogenic transcription factor by a chromosomal translocation between the EWSR1 gene and the ETS transcription factor family. However, the transcriptional mechanism of EWS-ETS fusion proteins is still unclear. To identify the transcriptional complexes of EWS-ETS fusion transcription factors, we applied a proximal labeling system called BioID in Ewing's sarcoma cells. We identified AHDC1 as a proximal protein of EWS-ETS fusion proteins. AHDC1 knockdown showed a reduced cell growth and transcriptional activity of EWS-FLI1. AHDC1 knockdown also reduced BRD4 and BRG1 protein levels, both known as interacting proteins of EWS-FLI1. Our results suggest that AHDC1 supports cell growth through EWS-FLI1. |
| 巻・号 | 17(10) |
| ページ | e0269077 |
| 公開日 | 2022-1-1 |
| DOI | 10.1371/journal.pone.0269077 |
| PII | PONE-D-22-13872 |
| PMID | 36194562 |
| PMC | PMC9531837 |
| MeSH | Cell Cycle Proteins / metabolism Child DNA DNA-Binding Proteins / genetics Gene Expression Regulation, Neoplastic Humans Nuclear Proteins / genetics Oncogene Proteins, Fusion / metabolism Proto-Oncogene Protein c-fli-1 / genetics Proto-Oncogene Protein c-fli-1 / metabolism RNA-Binding Protein EWS / genetics RNA-Binding Protein EWS / metabolism Sarcoma, Ewing* / pathology Transcription Factors / genetics Transcription Factors / metabolism |
| IF | 2.74 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |