Reference - Detail
| RRC ID | 81382 |
|---|---|
| Author | Song W, Zhang D, Mi J, Du W, Yang Y, Chen R, Tian C, Zhao X, Zou K. |
| Title | E-cadherin maintains the undifferentiated state of mouse spermatogonial progenitor cells via β-catenin. |
| Journal | Cell Biosci |
| Abstract |
BACKGROUND:Cadherins play a pivotal role in facilitating intercellular interactions between spermatogonial progenitor cells (SPCs) and their surrounding microenvironment. Specifically, E-cadherin serves as a cellular marker of SPCs in many species. Depletion of E-cadherin in mouse SPCs showed no obvious effect on SPCs homing and spermatogenesis. RESULTS:Here, we investigated the regulatory role of E-cadherin in regulating SPCs fate. Specific deletion of E-cadherin in germ cells was shown to promote SPCs differentiation, evidencing by reduced PLZF+ population and increased c-Kit+ population in mouse testes. E-cadherin loss down-regulated the expression level of β-catenin, leading to the reduced β-catenin in nuclear localization for transcriptional activity. Remarkably, increasing expression level of Cadherin-22 (CDH22) appeared specifically after E-cadherin deletion, indicating CDH22 played a synergistic effect with E-cadherin in SPCs. By searching for the binding partners of β-catenin, Lymphoid enhancer-binding factor 1 (LEF1), T-cell factor (TCF3), histone deacetylase 4 (HDAC4) and signal transducer and activator 3 (STAT3) were identified as suppressors of SPCs differentiation by regulating acetylation of differentiation genes with PLZF. CONCLUSIONS:Two surface markers of SPCs, E-cadherin and Cadherin-22, synergically maintain the undifferentiation of SPCs via the pivotal intermediate molecule β-catenin. LEF1, TCF3, STAT3 and HDAC4 were identified as co-regulatory factors of β-catenin in regulation of SPC fate. These observations revealed a novel regulatory pattern of cadherins on SPCs fate. |
| Volume | 12(1) |
| Pages | 141 |
| Published | 2022-9-1 |
| DOI | 10.1186/s13578-022-00880-w |
| PII | 10.1186/s13578-022-00880-w |
| PMID | 36050783 |
| PMC | PMC9434974 |
| Resource | |
| DNA material | CSII-EF-MCS-IRES2-Venus (RDB04384) pCMV-VSV-G-RSV-Rev (RDB04393) pCAG-HIVgp (RDB4394) |