| RRC ID |
81390
|
| Author |
Kimura M, Sekiguchi K, Okitsu S, Ushijima H, Tani H.
|
| Title |
A highly quantitative detection system for cell entry of human norovirus-like particles based on the complementation of NanoLuc luciferase.
|
| Journal |
Virology
|
| Abstract |
Human norovirus (HuNoV) is the common cause of acute gastroenteritis worldwide. Norovirus-like particles (VLPs) have been applied to analyze virus-host cell interactions. This study established a highly quantitative detection system for HuNoV VLP entry into live cells based on the NanoLuc luciferase complementation. First, we generated a HiBiT-tagged VLP and examined VLP-HiBiT entry into Vero cells expressing LgBiT. The entry was saturable and competed by a non-labeled VLP, indicating the VLP-specific entry into the host cells. Upon comparison of the VLP-HiBiT entry among some cell lines, Caco-2 cells were found to be the most susceptible. The VLP-HiBiT entry was unaffected by treatment at an acidic pH value above 3.0 and was significantly reduced by heat treatment at 75°C for more than 2 min. These results reflected those of previous studies, indicating the stability of VLPs and binding to histo-blood group antigens at various pH ranges and temperatures and cellular tropism.
|
| Volume |
573
|
| Pages |
23-28
|
| Published |
2022-8-1
|
| DOI |
10.1016/j.virol.2022.06.001
|
| PII |
S0042-6822(22)00087-3
|
| PMID |
35691115
|
| MeSH |
Animals
Caco-2 Cells
Chlorocebus aethiops
Humans
Luciferases / genetics
Luciferases / metabolism
Norovirus* / genetics
Vero Cells
Virus Internalization
|
| IF |
2.819
|
| Resource |
| DNA material |
pCAG-HIVgp (RDB04394)
pCMV-VSV-G-RSV-Rev (RDB04393) |
