論文 - 詳細
| RRC ID | 81390 |
|---|---|
| 著者 | Kimura M, Sekiguchi K, Okitsu S, Ushijima H, Tani H. |
| タイトル | A highly quantitative detection system for cell entry of human norovirus-like particles based on the complementation of NanoLuc luciferase. |
| ジャーナル | Virology |
| Abstract |
Human norovirus (HuNoV) is the common cause of acute gastroenteritis worldwide. Norovirus-like particles (VLPs) have been applied to analyze virus-host cell interactions. This study established a highly quantitative detection system for HuNoV VLP entry into live cells based on the NanoLuc luciferase complementation. First, we generated a HiBiT-tagged VLP and examined VLP-HiBiT entry into Vero cells expressing LgBiT. The entry was saturable and competed by a non-labeled VLP, indicating the VLP-specific entry into the host cells. Upon comparison of the VLP-HiBiT entry among some cell lines, Caco-2 cells were found to be the most susceptible. The VLP-HiBiT entry was unaffected by treatment at an acidic pH value above 3.0 and was significantly reduced by heat treatment at 75°C for more than 2 min. These results reflected those of previous studies, indicating the stability of VLPs and binding to histo-blood group antigens at various pH ranges and temperatures and cellular tropism. |
| 巻・号 | 573 |
| ページ | 23-28 |
| 公開日 | 2022-8-1 |
| DOI | 10.1016/j.virol.2022.06.001 |
| PII | S0042-6822(22)00087-3 |
| PMID | 35691115 |
| MeSH | Animals Caco-2 Cells Chlorocebus aethiops Humans Luciferases / genetics Luciferases / metabolism Norovirus* / genetics Vero Cells Virus Internalization |
| IF | 2.819 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCAG-HIVgp (RDB04394) pCMV-VSV-G-RSV-Rev (RDB04393) |