RRC ID 81418
Author Pedroni A, Dai YE, Lafouasse L, Chang W, Srivastava I, Del Vecchio L, Ampatzis K.
Title Neuroprotective gap-junction-mediated bystander transformations in the adult zebrafish spinal cord after injury.
Journal Nat Commun
Abstract The adult zebrafish spinal cord displays an impressive innate ability to regenerate after traumatic insults, yet the underlying adaptive cellular mechanisms remain elusive. Here, we show that while the cellular and tissue responses after injury are largely conserved among vertebrates, the large-size fast spinal zebrafish motoneurons are remarkably resilient by remaining viable and functional. We also reveal the dynamic changes in motoneuron glutamatergic input, excitability, and calcium signaling, and we underscore the critical role of calretinin (CR) in binding and buffering the intracellular calcium after injury. Importantly, we demonstrate the presence and the dynamics of a neuron-to-neuron bystander neuroprotective biochemical cooperation mediated through gap junction channels. Our findings support a model in which the intimate and dynamic interplay between glutamate signaling, calcium buffering, gap junction channels, and intercellular cooperation upholds cell survival and promotes the initiation of regeneration.
Volume 15(1)
Pages 4331
Published 2024-5-21
DOI 10.1038/s41467-024-48729-9
PII 10.1038/s41467-024-48729-9
PMID 38773121
PMC PMC11109231
MeSH Animals Calbindin 2 / metabolism Calcium / metabolism Calcium Signaling Cell Survival Gap Junctions* / metabolism Glutamic Acid / metabolism Motor Neurons* / metabolism Spinal Cord* / metabolism Spinal Cord Injuries* / metabolism Zebrafish* Zebrafish Proteins / genetics Zebrafish Proteins / metabolism
IF 12.121
Resource
Zebrafish Tg(chx10:GFP)