論文 - 詳細
| RRC ID | 81420 |
|---|---|
| 著者 | Saraswathy VM, Zhou L, Mokalled MH. |
| タイトル | Single-cell analysis of innate spinal cord regeneration identifies intersecting modes of neuronal repair. |
| ジャーナル | Nat Commun |
| Abstract |
Adult zebrafish have an innate ability to recover from severe spinal cord injury. Here, we report a comprehensive single nuclear RNA sequencing atlas that spans 6 weeks of regeneration. We identify cooperative roles for adult neurogenesis and neuronal plasticity during spinal cord repair. Neurogenesis of glutamatergic and GABAergic neurons restores the excitatory/inhibitory balance after injury. In addition, a transient population of injury-responsive neurons (iNeurons) show elevated plasticity 1 week post-injury. We found iNeurons are injury-surviving neurons that acquire a neuroblast-like gene expression signature after injury. CRISPR/Cas9 mutagenesis showed iNeurons are required for functional recovery and employ vesicular trafficking as an essential mechanism that underlies neuronal plasticity. This study provides a comprehensive resource of the cells and mechanisms that direct spinal cord regeneration and establishes zebrafish as a model of plasticity-driven neural repair. |
| 巻・号 | 15(1) |
| ページ | 6808 |
| 公開日 | 2024-8-15 |
| DOI | 10.1038/s41467-024-50628-y |
| PII | 10.1038/s41467-024-50628-y |
| PMID | 39147780 |
| PMC | PMC11327264 |
| MeSH | Animals Animals, Genetically Modified CRISPR-Cas Systems Disease Models, Animal GABAergic Neurons / metabolism Nerve Regeneration / physiology Neurogenesis* / genetics Neuronal Plasticity* / physiology Neurons / metabolism Neurons / physiology Recovery of Function Single-Cell Analysis* Spinal Cord* / metabolism Spinal Cord Injuries* / metabolism Spinal Cord Regeneration* Zebrafish* |
| IF | 12.121 |
| リソース情報 | |
| ゼブラフィッシュ | Tg(vglut2a:lRl-GFP) Tg(gad1b:GFP) Tg(CM-isl1:GFP)rw0 |