Reference - Detail
RRC ID | 81420 |
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Author | Saraswathy VM, Zhou L, Mokalled MH. |
Title | Single-cell analysis of innate spinal cord regeneration identifies intersecting modes of neuronal repair. |
Journal | Nat Commun |
Abstract |
Adult zebrafish have an innate ability to recover from severe spinal cord injury. Here, we report a comprehensive single nuclear RNA sequencing atlas that spans 6 weeks of regeneration. We identify cooperative roles for adult neurogenesis and neuronal plasticity during spinal cord repair. Neurogenesis of glutamatergic and GABAergic neurons restores the excitatory/inhibitory balance after injury. In addition, a transient population of injury-responsive neurons (iNeurons) show elevated plasticity 1 week post-injury. We found iNeurons are injury-surviving neurons that acquire a neuroblast-like gene expression signature after injury. CRISPR/Cas9 mutagenesis showed iNeurons are required for functional recovery and employ vesicular trafficking as an essential mechanism that underlies neuronal plasticity. This study provides a comprehensive resource of the cells and mechanisms that direct spinal cord regeneration and establishes zebrafish as a model of plasticity-driven neural repair. |
Volume | 15(1) |
Pages | 6808 |
Published | 2024-8-15 |
DOI | 10.1038/s41467-024-50628-y |
PII | 10.1038/s41467-024-50628-y |
PMID | 39147780 |
PMC | PMC11327264 |
MeSH | Animals Animals, Genetically Modified CRISPR-Cas Systems Disease Models, Animal GABAergic Neurons / metabolism Nerve Regeneration / physiology Neurogenesis* / genetics Neuronal Plasticity* / physiology Neurons / metabolism Neurons / physiology Recovery of Function Single-Cell Analysis* Spinal Cord* / metabolism Spinal Cord Injuries* / metabolism Spinal Cord Regeneration* Zebrafish* |
IF | 12.121 |
Resource | |
Zebrafish | Tg(vglut2a:lRl-GFP) Tg(gad1b:GFP) |