Reference - Detail
| RRC ID | 81425 |
|---|---|
| Author | Filippo Binci, Elisabetta Offer, Andrea Crosino, Ivan Sciascia, Jürgen Kleine-Vehn, Andrea Genre, Marco Giovannetti, Lorella Navazio |
| Title | Spatially and temporally distinct Ca2+ changes in Lotus japonicus roots orient fungal-triggered signalling pathways towards symbiosis or immunity |
| Journal | Journal of Experimental Botany |
| Abstract |
Plants activate an immune or symbiotic response depending on the detection of distinct signals from root-interacting microbes. Both signalling cascades involve Ca2+ as a central mediator of early signal transduction. In this study, we combined aequorin- and cameleon-based methods to dissect the changes in cytosolic and nuclear Ca2+ concentration caused by different chitin-derived fungal elicitors in Lotus japonicus roots. Our quantitative analyses highlighted the dual character of the evoked Ca2+ responses taking advantage of the comparison between different genetic backgrounds: an initial Ca2+ influx, dependent on the LysM receptor CERK6 and independent of the common symbiotic signalling pathway (CSSP), is followed by a second CSSP-dependent and CERK6-independent phase, that corresponds to the well-known perinuclear/nuclear Ca2+ spiking. We show that the expression of immunity marker genes correlates with the amplitude of the first Ca2+ change, depends on elicitor concentration, and is controlled by Ca2+ storage in the vacuole. Our findings provide an insight into the Ca2+-mediated signalling mechanisms discriminating plant immunity- and symbiosis-related pathways in the context of their simultaneous activation by single fungal elicitors. |
| Volume | 75 |
| Pages | 605-619 |
| Published | 2024-1-10 |
| DOI | 10.1093/jxb/erad360 |
| PMID | 37712520 |
| MeSH | Calcium / metabolism Gene Expression Regulation, Plant Lotus* / metabolism Mycorrhizae* / physiology Plant Proteins / genetics Plant Proteins / metabolism Plant Roots / metabolism Signal Transduction Symbiosis / genetics |
| IF | 5.908 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 25 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Lotus / Glycine | Gifu B-129 |