RRC ID 81474
Author Nguyen YDH, Pham TLA, Nishihara T, Kamei K, Tran DB.
Title Depletion of lipid storage droplet-1 delays endoreplication progression and induces cell death in Drosophila salivary gland.
Journal Arch Insect Biochem Physiol
Abstract Perilipins are evolutionarily conserved from insects to mammals. Drosophila lipid storage droplet-1 (LSD-1) is a lipid storage droplet membrane surface-binding protein family member and a counterpart to mammalian perilipin 1 and is known to play a role in lipolysis. However, the function of LSD-1 during specific tissue development remains under investigation. This study demonstrated the role of LSD-1 in salivary gland development. Knockdown of Lsd-1 in the salivary gland was established using the GAL4/UAS system. The third-instar larvae of knockdown flies had small salivary glands containing cells with smaller nuclei. The null mutant Drosophila also showed the same phenotype. The depletion of LSD-1 expression induced a delay of endoreplication due to decreasing CycE expression and increasing DNA damage. Lsd-1 genetically interacted with Myc in the third-instar larvae. These results demonstrate that LSD-1 is involved in cell cycle and cell death programs in the salivary gland, providing novel insight into the effects of LSD-1 in regulating salivary gland development and the interaction between LSD-1 and Myc.
Volume 116(3)
Pages e22132
Published 2024-7-1
DOI 10.1002/arch.22132
PMID 38993002
MeSH Animals Cell Death* DNA Replication DNA-Binding Proteins Drosophila / genetics Drosophila / metabolism Drosophila Proteins* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / genetics Drosophila melanogaster / growth & development Drosophila melanogaster / metabolism Larva* / genetics Larva* / growth & development Larva* / metabolism Oxidoreductases, N-Demethylating Proto-Oncogene Proteins c-myc / genetics Proto-Oncogene Proteins c-myc / metabolism Salivary Glands* / cytology Salivary Glands* / metabolism Transcription Factors
Resource
Drosophila DGRC#108479