| RRC ID |
81513
|
| Author |
Kanai Y, Onishi M, Yoshida Y, Kotaki T, Minami S, Nouda R, Yamasaki M, Enoki Y, Kobayashi T.
|
| Title |
Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene.
|
| Journal |
J Virol
|
| Abstract |
The stabilities of transgenes in RNA virus vectors differ between the genes of interest, but the molecular mechanisms determining genetic stability remain unknown. This study demonstrated that the stability of a transgene was affected by the nucleotide composition, and altering the codon usage of transgenes to resemble that of the viral genome significantly increased transgene stability in double-stranded RNA virus vectors. The virus-like codon modification strategy enabled generation of stable rotavirus and mammalian orthoreovirus vectors, which could be developed as machinery for gene delivery to the intestines and/or respiratory organs. This technology has further potential to be expanded to other RNA viruses.
|
| Volume |
97(10)
|
| Pages |
e0049223
|
| Published |
2023-10-31
|
| DOI |
10.1128/jvi.00492-23
|
| PMID |
37732784
|
| PMC |
PMC10617491
|
| MeSH |
Animals
Codon / genetics
Double Stranded RNA Viruses* / genetics
Genetic Engineering
Genetic Vectors / genetics
Genome, Viral
Mammals / genetics
RNA Viruses* / genetics
Transgenes
|
| Resource |
| Human pathogenic viruses |
|
