論文 - 詳細
| RRC ID | 81513 |
|---|---|
| 著者 | Kanai Y, Onishi M, Yoshida Y, Kotaki T, Minami S, Nouda R, Yamasaki M, Enoki Y, Kobayashi T. |
| タイトル | Genetic engineering strategy for generating a stable dsRNA virus vector using a virus-like codon-modified transgene. |
| ジャーナル | J Virol |
| Abstract |
The stabilities of transgenes in RNA virus vectors differ between the genes of interest, but the molecular mechanisms determining genetic stability remain unknown. This study demonstrated that the stability of a transgene was affected by the nucleotide composition, and altering the codon usage of transgenes to resemble that of the viral genome significantly increased transgene stability in double-stranded RNA virus vectors. The virus-like codon modification strategy enabled generation of stable rotavirus and mammalian orthoreovirus vectors, which could be developed as machinery for gene delivery to the intestines and/or respiratory organs. This technology has further potential to be expanded to other RNA viruses. |
| 巻・号 | 97(10) |
| ページ | e0049223 |
| 公開日 | 2023-10-31 |
| DOI | 10.1128/jvi.00492-23 |
| PMID | 37732784 |
| PMC | PMC10617491 |
| MeSH | Animals Codon / genetics Double Stranded RNA Viruses* / genetics Genetic Engineering Genetic Vectors / genetics Genome, Viral Mammals / genetics RNA Viruses* / genetics Transgenes |
| IF | 4.501 |
| オルトメトリクス指標 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
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