RRC ID 81561
Author Koichi Sugimoto, Eiichiro Ono, Tamaki Inaba, Takehiko Tsukahara, Kenji Matsui, Manabu Horikawa, Hiromi Toyonaga, Kohki Fujikawa, Tsukiho Osawa, Shunichi Homma, Yoshikazu Kiriiwa, Ippei Ohmura, Atsushi Miyagawa, Hatsuo Yamamura, Mikio Fujii, Rika Ozawa, Bunta Watanabe, Kenji Miura, Hiroshi Ezura, Toshiyuki Ohnishi, Junji Takabayashi
Title Identification of a tomato UDP-arabinosyltransferase for airborne volatile reception
Journal Nature Communications
Abstract Volatiles from herbivore-infested plants function as a chemical warning of future herbivory for neighboring plants. (Z)-3-Hexenol emitted from tomato plants infested by common cutworms is taken up by uninfested plants and converted to (Z)-3-hexenyl β-vicianoside (HexVic). Here we show that a wild tomato species (Solanum pennellii) shows limited HexVic accumulation compared to a domesticated tomato species (Solanum lycopersicum) after (Z)-3-hexenol exposure. Common cutworms grow better on an introgression line containing anS. pennelliichromosome 11 segment that impairs HexVic accumulation, suggesting that (Z)-3-hexenol diglycosylation is involved in the defense of tomato against herbivory. We finally reveal that HexVic accumulation is genetically associated with a uridine diphosphate-glycosyltransferase (UGT) gene cluster that harborsUGT91R1on chromosome 11. Biochemical and transgenic analyses of UGT91R1 show that it preferentially catalyzes (Z)-3-hexenyl β-d-glucopyranoside arabinosylation to produce HexVicin planta.
Volume 14
Published 2023-2-8
DOI 10.1038/s41467-023-36381-8
PMID 36755045
PMC PMC9908901
MeSH Glycosyltransferases / genetics Herbivory Pentosyltransferases Solanum* Solanum lycopersicum* / genetics Volatile Organic Compounds* / analysis
IF 12.121
Resource
Tomato TOMJPF00009 TOMJPF00010 TOMJPF00011 TOMJPF00008