Reference - Detail
| RRC ID | 81641 |
|---|---|
| Author | Kashojiya S, Lu Y, Takayama M, Komatsu H, Minh LHT, Nishida K, Shirasawa K, Miura K, Nonaka S, Masuda JI, Kondo A, Ezura H, Ariizumi T. |
| Title | Modification of tomato breeding traits and plant hormone signaling by target-AID, the genome-editing system inducing efficient nucleotide substitution. |
| Journal | Hortic Res |
| Abstract |
Target activation-induced cytidine deaminase (Target-AID), a novel CRISPR/Cas9-based genome-editing tool, confers the base-editing capability on the Cas9 genome-editing system. It involves the fusion of cytidine deaminase (CDA), which catalyzes cytidine (C) to uridine (U) substitutions, to the mutated nickase-type nCas9 or deactivated-type dCas9. To confirm and extend the applicability of the Target-AID genome-editing system in tomatoes (Solanum lycopersicum L.), we transformed the model tomato cultivar "Micro-Tom" and commercial tomato cultivars using this system by targeting SlDELLA, which encodes a negative regulator of the plant phytohormone gibberellic acid (GA) signaling pathway. We confirmed that the nucleotide substitutions were induced by the Target-AID system, and we isolated mutants showing high GA sensitivity in both "Micro-Tom" and the commercial cultivars. Moreover, by successfully applying this system to ETHYLENE RECEPTOR 1 (SlETR1) with single sgRNA targeting, double sgRNA targeting, as well as dual-targeting of both SlETR1 and SlETR2 with a single sgRNA, we demonstrated that the Target-AID genome-editing system is a promising tool for molecular breeding in tomato crops. This study highlights an important aspect of the scientific and agricultural potential of the combinatorial use of the Target-AID and other base-editing systems. |
| Volume | 9 |
| Published | 2022-1-19 |
| DOI | 10.1093/hr/uhab004 |
| PII | 6511229 |
| PMID | 35043178 |
| PMC | PMC8795821 |
| IF | 5.404 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | News |
| Total number of mentions | 6 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Tomato | TOMJPF00001 TOMJPF0017 |