| RRC ID |
81681
|
| 著者 |
Hu X, Roy SR, Jin C, Li G, Zhang Q, Asano N, Asahina S, Kajiwara T, Takahara A, Feng B, Aoki K, Xu C, Zhang Y.
|
| タイトル |
Control cell migration by engineering integrin ligand assembly.
|
| ジャーナル |
Nat Commun
|
| Abstract |
Advances in mechanistic understanding of integrin-mediated adhesion highlight the importance of precise control of ligand presentation in directing cell migration. Top-down nanopatterning limited the spatial presentation to sub-micron placing restrictions on both fundamental study and biomedical applications. To break the constraint, here we propose a bottom-up nanofabrication strategy to enhance the spatial resolution to the molecular level using simple formulation that is applicable as treatment agent. Via self-assembly and co-assembly, precise control of ligand presentation is succeeded by varying the proportions of assembling ligand and nonfunctional peptide. Assembled nanofilaments fulfill multi-functions exerting enhancement to suppression effect on cell migration with tunable amplitudes. Self-assembled nanofilaments possessing by far the highest ligand density prevent integrin/actin disassembly at cell rear, which expands the perspective of ligand-density-dependent-modulation, revealing valuable inputs to therapeutic innovations in tumor metastasis.
|
| 巻・号 |
13(1)
|
| ページ |
5002
|
| 公開日 |
2022-8-25
|
| DOI |
10.1038/s41467-022-32686-2
|
| PII |
10.1038/s41467-022-32686-2
|
| PMID |
36008449
|
| PMC |
PMC9411606
|
| MeSH |
Cell Adhesion / physiology
Cell Movement / physiology
Integrins* / metabolism
Ligands
Protein Binding
|
| IF |
12.121
|
| リソース情報 |
| ヒト・動物細胞 |
HuH-7(RCB1366)
Hep G2(RCB1886)
MKN1(RCB1003)
MCF7(RCB1904)
HeLa(RCB0007) |
