論文 - 詳細
| RRC ID | 81690 |
|---|---|
| 著者 | Muguruma M, Asaoka M, Teraoka S, Miyahara K, Kawate T, Ishikawa T. |
| タイトル | β-Tubulin Isoforms Related to Docetaxel Sensitivity in 2D and 3D Cultured TNBC Cell Lines. |
| ジャーナル | Anticancer Res |
| Abstract |
BACKGROUND/AIM:We previously reported that the half maximal inhibitory concentration (IC50) values of cisplatin and epirubicin correlated in 13 triple-negative breast cancer (TNBC) cell lines between two-dimensional (2D) and three-dimensional (3D) culture methods. However, the IC50 values of docetaxel (DTX) did not correlate between the two culture methods. We hypothesized that this non-correlation is partly associated with differences in expression of the β-tubulin isoform, the target molecule of DTX and in morphology depending on the culture method. MATERIALS AND METHODS:We investigated the expression levels of β-tubulin isoforms by real-time polymerase chain reaction and morphology of spheroid formation in the 13 TNBC cell lines cultured using the 2D and 3D culture methods. RESULTS:Tubulin β class I (TUBB) expression levels were negatively correlated with the IC50 value of DTX in the 2D culture method (R=-0.360), whereas tubulin β class IIa (TUBB2a) expression levels were positively correlated in the 3D culture method (R=0.398). There was no significant difference in the expression levels of β-tubulin isoforms between the 2D and 3D culture methods. The spheroids were classified morphologically into three types: round, mass, and grape-like. However, no clear association was found between DTX sensitivity and morphology. CONCLUSION:The non-correlation of the IC50 values of DTX between the 2D and 3D culture methods does not appear to be due to the changes in β-tubulin isoforms. Morphology in the 3D culture method may play some role in drug sensitivity. |
| 巻・号 | 42(10) |
| ページ | 4735-4742 |
| 公開日 | 2022-10-1 |
| DOI | 10.21873/anticanres.15978 |
| PII | 42/10/4735 |
| PMID | 36191981 |
| MeSH | Cell Line Cell Line, Tumor Cisplatin Docetaxel / pharmacology Epirubicin / pharmacology Humans Protein Isoforms Triple Negative Breast Neoplasms* / drug therapy Tubulin* / metabolism |
| IF | 1.994 |
| リソース情報 | |
| ヒト・動物細胞 | MDA-MB-453(RCB1192) |