論文 - 詳細
| RRC ID | 81692 |
|---|---|
| 著者 | Sudo H, Tonoyama Y, Ikebe E, Hasegawa H, Iha H, Ishida YI. |
| タイトル | Proteomic analysis of adult T-cell leukemia/lymphoma: A biomarker identification strategy based on preparation and in-solution digestion methods of total proteins. |
| ジャーナル | Leuk Res |
| Abstract |
Adult T-cell leukemia/lymphoma (ATL), caused by human T-cell leukemia virus type-1 (HTLV-1) infection, is a malignant hematologic cancer that remains difficult to cure. We herein established a biomarker identification strategy based on the total cell proteomics of cultured ATL cells to search for novel ATL biomarkers. Four protocols with a combination of selected conditions based on lysis buffers and addition agents for total cell proteomics were used for a differential analysis between the ATL cell group (consisting of 11 cell lines), HTLV-1-infected cell group (consisting of 6 cell lines), and HTLV-1-negative cell group (consisting of 6 cell lines). In the analysis, we identified 24 and 27 proteins that were significantly increased (ratio ≥2.0, p < 0.05) and decreased (ratio ≤ 0.5, p < 0.05), respectively, in the ATL group. Previously reported CCL3 and CD30/TNFRSF8 were confirmed to be among significantly increased proteins. Furthermore, correlation analysis between identified proteins and Tax suggested that RASSF2 and GORASP2 were candidates of novel Tax-regulated factors. The biomarker identification strategy established herein is expected to contribute to the identification of biomarkers for ATL and other diseases. |
| 巻・号 | 138 |
| ページ | 107454 |
| 公開日 | 2024-3-1 |
| DOI | 10.1016/j.leukres.2024.107454 |
| PII | S0145-2126(24)00020-1 |
| PMID | 38452534 |
| MeSH | Adult Biomarkers Digestion Gene Products, tax / metabolism Golgi Matrix Proteins Human T-lymphotropic virus 1* / metabolism Humans Leukemia-Lymphoma, Adult T-Cell* Lymphoma* Proteomics |
| IF | 2.214 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | ATN-1(RCB1440) ILT-Mat(RCB0475) TL-Mor(RCB1881) |