| RRC ID |
81692
|
| 著者 |
Sudo H, Tonoyama Y, Ikebe E, Hasegawa H, Iha H, Ishida YI.
|
| タイトル |
Proteomic analysis of adult T-cell leukemia/lymphoma: A biomarker identification strategy based on preparation and in-solution digestion methods of total proteins.
|
| ジャーナル |
Leuk Res
|
| Abstract |
Adult T-cell leukemia/lymphoma (ATL), caused by human T-cell leukemia virus type-1 (HTLV-1) infection, is a malignant hematologic cancer that remains difficult to cure. We herein established a biomarker identification strategy based on the total cell proteomics of cultured ATL cells to search for novel ATL biomarkers. Four protocols with a combination of selected conditions based on lysis buffers and addition agents for total cell proteomics were used for a differential analysis between the ATL cell group (consisting of 11 cell lines), HTLV-1-infected cell group (consisting of 6 cell lines), and HTLV-1-negative cell group (consisting of 6 cell lines). In the analysis, we identified 24 and 27 proteins that were significantly increased (ratio ≥2.0, p < 0.05) and decreased (ratio ≤ 0.5, p < 0.05), respectively, in the ATL group. Previously reported CCL3 and CD30/TNFRSF8 were confirmed to be among significantly increased proteins. Furthermore, correlation analysis between identified proteins and Tax suggested that RASSF2 and GORASP2 were candidates of novel Tax-regulated factors. The biomarker identification strategy established herein is expected to contribute to the identification of biomarkers for ATL and other diseases.
|
| 巻・号 |
138
|
| ページ |
107454
|
| 公開日 |
2024-3-1
|
| DOI |
10.1016/j.leukres.2024.107454
|
| PII |
S0145-2126(24)00020-1
|
| PMID |
38452534
|
| MeSH |
Adult
Biomarkers
Digestion
Gene Products, tax / metabolism
Golgi Matrix Proteins
Human T-lymphotropic virus 1* / metabolism
Humans
Leukemia-Lymphoma, Adult T-Cell*
Lymphoma*
Proteomics
|
| IF |
2.214
|
| リソース情報 |
| ヒト・動物細胞 |
ATN-1(RCB1440)
ILT-Mat(RCB0475)
TL-Mor(RCB1881) |
