RRC ID 81695
Author Maruyama K, Miyazaki S, Kobayashi R, Hikita H, Tsubone T, Ohnuma K.
Title The migration pattern of cells during the mesoderm and endoderm differentiation from human pluripotent stem cells.
Journal In Vitro Cell Dev Biol Anim
Abstract Gastrulation is the first major differentiation process in animal embryos. However, the dynamics of human gastrulation remain mostly unknown owing to the ethical limitations. We studied the dynamics of the mesoderm and endoderm cell differentiation from human pluripotent stem cells for insight into the cellular dynamics of human gastrulation. Human pluripotent stem cells have properties similar to those of the epiblast, which gives rise to the three germ layers. The mesoderm and endoderm were induced with more than 75% purity from human induced pluripotent stem cells. Single-cell dynamics of pluripotent stem cell-derived mesoderm and endoderm cells were traced using time-lapse imaging. Both mesoderm and endoderm cells migrate randomly, accompanied by short-term directional persistence. No substantial differences were detected between mesoderm and endoderm migration. Computer simulations created using the measured parameters revealed that random movement and external force, such as the spread out of cells from the primitive streak area, mimicked the homogeneous discoidal germ layer formation. These results were consistent with the development of amniotes, which suggests the effectiveness of human pluripotent stem cells as a good model for studying human embryogenesis.
Volume 60(5)
Pages 535-543
Published 2024-5-1
DOI 10.1007/s11626-024-00904-4
PII 10.1007/s11626-024-00904-4
PMID 38656570
MeSH Cell Differentiation* Cell Movement* Computer Simulation Endoderm* / cytology Humans Mesoderm* / cytology Pluripotent Stem Cells* / cytology
Resource
Human and Animal Cells 201B7(HPS0063)