| RRC ID |
81697
|
| Author |
Mitsutani M, Yokoyama M, Hano H, Morita A, Matsushita M, Tagami T, Moriyama K.
|
| Title |
Growth hormone is involved in GATA1 gene expression via STAT5B in human erythroleukemia and monocytic cell lines.
|
| Journal |
Blood Cells Mol Dis
|
| Abstract |
GATAs are a family of transcription factors consisting of six members. Particularly, GATA1 and GATA2 have been reported to promote the development of erythrocytes, megakaryocytes, eosinophils, and mast cells. However, little information is available on the extracellular ligands that promote GATA1 expression. We evaluated whether growth hormone (GH) is an extracellular stimulator that participates in the signal transduction of GATAs, focusing on GATA1 expression in hematopoietic cell lineages. We used a reporter assay, RT-PCR, real-time quantitative PCR, and western blotting to evaluate GH-induced expression of GATA1 and GATA2 in the human erythroleukemic cell line K562 and the non-erythroid cell line U937. GATA1 expression in these hematopoietic cell lines increased at the transcriptional and protein levels in the presence of GH, and was inhibited by a STAT5 specific inhibitor. Cells transfected with activated STAT5B showed increased expression of GATA1. We identified functional STAT5B consensus sequences as binding site-158 bp from the transcription starting site in the GATA1 promoter region. These results suggest that GH directly induces GATA1 expression via GHR/JAK/STAT5 and is related to hematopoietic cell proliferation.
|
| Volume |
110
|
| Pages |
102894
|
| Published |
2025-2-1
|
| DOI |
10.1016/j.bcmd.2024.102894
|
| PII |
S1079-9796(24)00072-X
|
| PMID |
39303396
|
| MeSH |
GATA1 Transcription Factor* / genetics
GATA1 Transcription Factor* / metabolism
GATA2 Transcription Factor / genetics
GATA2 Transcription Factor / metabolism
Gene Expression Regulation, Leukemic
Growth Hormone / metabolism
Growth Hormone / pharmacology
Humans
K562 Cells
Leukemia, Erythroblastic, Acute* / genetics
Leukemia, Erythroblastic, Acute* / metabolism
Leukemia, Erythroblastic, Acute* / pathology
Monocytes* / metabolism
Promoter Regions, Genetic
STAT5 Transcription Factor* / genetics
STAT5 Transcription Factor* / metabolism
Signal Transduction
U937 Cells
|
| IF |
2.46
|
| Resource |
| Human and Animal Cells |
Hep G2(RCB1886) |
