論文 - 詳細
| RRC ID | 81716 |
|---|---|
| 著者 | Shibuya K, Nozawa A, Takahashi C, Sawasaki T. |
| タイトル | A chemical approach to extend flower longevity of Japanese morning glory via inhibition of master senescence regulator EPHEMERAL1. |
| ジャーナル | Nat Plants |
| Abstract |
Petal senescence in flowering plants is a type of programmed cell death with highly regulated onset and progression. A NAM/ATAF1,2/CUC2 transcription factor, EPHEMERAL1 (EPH1), has been identified as a key regulator of petal senescence in Japanese morning glory (Ipomoea nil). Here we used a novel chemical approach to delay petal senescence in Japanese morning glory by inhibiting the DNA-binding activity of EPH1. A cell-free high-throughput screening system and subsequent bioassays found two tetrafluorophthalimide-based compounds, Everlastin1 and Everlastin2, that inhibited the EPH1-DNA interaction and delayed petal senescence. The inhibitory mechanism was due to the suppression of EPH1 dimerization. RNA-sequencing analysis revealed that the chemical treatment strongly suppressed the expression of programmed cell death- and autophagy-related genes. These results suggest that a chemical approach targeting a transcription factor can regulate petal senescence. |
| 巻・号 | 10(9) |
| ページ | 1377-1388 |
| 公開日 | 2024-9-1 |
| DOI | 10.1038/s41477-024-01767-z |
| PII | 10.1038/s41477-024-01767-z |
| PMID | 39209993 |
| MeSH | Flowers* / drug effects Flowers* / genetics Gene Expression Regulation, Plant Ipomoea nil* / drug effects Ipomoea nil* / genetics Ipomoea nil* / metabolism Ipomoea nil* / physiology Plant Proteins* / genetics Plant Proteins* / metabolism Plant Senescence / genetics Transcription Factors* / genetics Transcription Factors* / metabolism |
| IF | 13.256 |
| リソース情報 | |
| アサガオ | Q0079 AK77 |