| RRC ID |
81767
|
| Author |
Takeguchi T, Kai H, Takeshita M, Miura M, Ogawa K, Nishiyama K, Yamasaki M.
|
| Title |
Koji Mold-derived Lipids Disrupt the Intracellular Redox State by Decreasing the GPx4 and Intracellular Glutathione Levels, Promoting Membrane Lipid Peroxidation, and Inducing Ferroptosis in HL-60 Cells.
|
| Journal |
J Oleo Sci
|
| Abstract |
In this study, we evaluated the cancer cell killing activity of koji mold-derived extracts using several solvents. The koji mold lipid extract (KML) exhibited potent cytotoxicity against a human leukemia cell line. Fractionation of the KML via silica gel chromatography revealed the presence of active components in fraction (Fr.) 6. Cytotoxic effects of Fr. 6 were inhibited by the ferroptosis inhibitors, ferrostatin-1 and SRS11-92, and the iron chelator, deferoxamine. Interestingly, ferroptosis inhibitors failed to prevent the KML-induced cell death. Fr. 6 decreased the expression of glutathione peroxidase 4 (GPx4) and increased the level of peroxidized plasma membrane lipids. Furthermore, Fr. 6 decreased the intracellular glutathione levels. Overall, our results suggest that Fr. 6 included in KML induces ferroptosis in HL-60 cells.
|
| Volume |
73(7)
|
| Pages |
991-999
|
| Published |
2024-1-1
|
| DOI |
10.5650/jos.ess24043
|
| PMID |
38945927
|
| MeSH |
Cyclohexylamines / pharmacology
Deferoxamine / pharmacology
Ferroptosis* / drug effects
Glutathione* / metabolism
HL-60 Cells
Humans
Iron Chelating Agents / pharmacology
Lipid Peroxidation* / drug effects
Lipids
Membrane Lipids / metabolism
Oxidation-Reduction* / drug effects
Phenylenediamines / pharmacology
Phospholipid Hydroperoxide Glutathione Peroxidase* / metabolism
|
| IF |
1.304
|
| Resource |
| Human and Animal Cells |
HL-60 |
