論文 - 詳細
| RRC ID | 81796 |
|---|---|
| 著者 | Furukawa H, Kimura Y, Inaba H, Matsuura K. |
| タイトル | A supramolecular system mimicking the infection process of an enveloped virus through membrane fusion. |
| ジャーナル | Sci Rep |
| Abstract |
Membrane fusion is an essential step for the entry of enveloped viruses, such as human immunodeficiency virus and influenza virus, into the host cell, often triggered by the binding of membrane proteins on the viral envelope to host cell membrane. Recently, external stimuli was shown to trigger membrane fusion in an artificial system. Direct observation of artificial membrane fusion using a giant unilamellar vesicle (GUV), which is similar in size to a cell, is useful as a biological model system. However, there are no model systems for studying membrane fusion of enveloped viruses with host cells. Here, we report a supramolecular model system for viral entry into a GUV or cell through membrane fusion. The system was constructed by complexing a cationic lipid bilayer on an anionic artificial viral capsid, self-assembled from viral β-annulus peptides. We demonstrate that the cationic enveloped artificial viral capsid electrostatically interacts with the anionic GUV or cell, and the capsid enters the GUV or cell through membrane fusion. The model system established in this study will be important for analyzing membrane fusion during infection of a natural virus. |
| 巻・号 | 13(1) |
| ページ | 19934 |
| 公開日 | 2023-11-15 |
| DOI | 10.1038/s41598-023-47347-7 |
| PII | 10.1038/s41598-023-47347-7 |
| PMID | 37968508 |
| PMC | PMC10651892 |
| MeSH | Capsid Proteins / metabolism Humans Membrane Fusion* Peptides / metabolism Virus Internalization Viruses* / metabolism |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 10 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |