RRC ID |
81811
|
Author |
Fujiyoshi J, Yamaza H, Sonoda S, Yuniartha R, Ihara K, Nonaka K, Taguchi T, Ohga S, Yamaza T.
|
Title |
Therapeutic potential of hepatocyte-like-cells converted from stem cells from human exfoliated deciduous teeth in fulminant Wilson's disease.
|
Journal |
Sci Rep
|
Abstract |
Wilson's disease (WD) is an inherited metabolic disease arising from ATPase copper transporting beta gene (ATP7B) mutation. Orthotoropic liver transplantation is the only radical treatment of fulminant WD, although appropriate donors are lacking at the onset of emergency. Given the hepatogenic capacity and tissue-integration/reconstruction ability in the liver of stem cells from human exfoliated deciduous teeth (SHED), SHED have been proposed as a source for curing liver diseases. We hypothesized the therapeutic potential of SHED and SHED-converted hepatocyte-like- cells (SHED-Heps) for fulminant WD. SHED and SHED-Heps were transplanted into WD model Atp7b-mutated Long-Evans Cinnamon (LEC) rats received copper overloading to induce a lethal fulminant liver failure. Due to the superior copper tolerance via ATP7B, SHED-Hep transplantation gave more prolonged life-span of fulminant LEC rats than SHED transplantation. The integrated ATP7B-expressing SHED-Heps showed more therapeutic effects on to restoring the hepatic dysfunction and tissue damages in the recipient liver than the integrated naïve SHED without ATP7B expression. Moreover, SHED-Heps could reduce copper-induced oxidative stress via ATP7B- independent stanniocalcin 1 secretion in the fulminant LEC rats, suggesting a possible role for paracrine effect of the integrated SHED-Heps. Taken together, SHED-Heps offer a potential of functional restoring, bridging, and preventive approaches for treating fulminant WD.
|
Volume |
9(1)
|
Pages |
1535
|
Published |
2019-2-7
|
DOI |
10.1038/s41598-018-38275-y
|
PII |
10.1038/s41598-018-38275-y
|
PMID |
30733544
|
PMC |
PMC6367569
|
MeSH |
Animals
Cell Differentiation
Copper / toxicity
Copper-Transporting ATPases / antagonists & inhibitors
Copper-Transporting ATPases / genetics
Copper-Transporting ATPases / metabolism
Disease Models, Animal
Glycoproteins / metabolism
Hepatocytes / cytology
Hepatocytes / metabolism
Hepatocytes / transplantation*
Hepatolenticular Degeneration / mortality
Hepatolenticular Degeneration / pathology
Hepatolenticular Degeneration / therapy*
Humans
Intercellular Signaling Peptides and Proteins / pharmacology
Oxidative Stress / drug effects
Paracrine Communication
RNA Interference
RNA, Small Interfering / metabolism
Rats
Rats, Inbred LEC
Stem Cells / cytology*
Stem Cells / metabolism
Survival Rate
Tooth, Deciduous / cytology*
|
IF |
3.998
|
Resource |
Human and Animal Cells |
Hep G2(RCB1886) |