RRC ID |
81854
|
Author |
Mouawad C, Awad MK, Liegeois S, Ferrandon D, Sanchis-Borja V, El Chamy L.
|
Title |
The NF-κB factor Relish is essential for the epithelial defenses protecting against δ-endotoxin dependent effects of Bacillus thuringiensis israelensis infection in the Drosophila model.
|
Journal |
Res Microbiol
|
Abstract |
Bacillus thuringiensis israelensis is largely regarded as the most selective, safe and ecofriendly biopesticide used for the control of insect vectors of human diseases. Bti enthomopathogenicity relies on the Cry and Cyt δ-endotoxins, produced as crystalline inclusions during sporulation. Insecticidal selectivity of Bti is mainly ascribed to the binding of the Cry toxins to receptors in the gut of target insects. However, the contribution of epithelial defenses in limiting Bti side effects in non-target species remains largely unexplored. Here, taking advantage of the genetically tractable Drosophila melanogaster model and its amenability for deciphering highly conserved innate immune defenses, we unravel a central role of the NF-κB factor Relish in the protection against the effects of ingested Bti spores in a non-susceptible host. Intriguingly, our data indicate that the Bti-induced Relish response is independent of its canonical activation downstream of peptidoglycan sensing and does not involve its longstanding role in the regulation of antimicrobial peptides encoding genes. In contrast, our data highlight a novel enterocyte specific function of Relish that is essential for preventing general septicemia following Bti oral infections strictly when producing δ-endotoxins. Altogether, our data provide novel insights into Bti-hosts interactions of prominent interest for the optimization and sustainability of insects' biocontrol strategies.
|
Volume |
174(6)
|
Pages |
104089
|
Published |
2023-1-1
|
DOI |
10.1016/j.resmic.2023.104089
|
PII |
S0923-2508(23)00064-5
|
PMID |
37348743
|
MeSH |
Animals
Bacillus thuringiensis* / genetics
Bacillus thuringiensis Toxins / metabolism
Bacterial Proteins / genetics
Bacterial Proteins / metabolism
Drosophila / metabolism
Drosophila melanogaster / metabolism
Endotoxins* / genetics
Endotoxins* / metabolism
Endotoxins* / pharmacology
Hemolysin Proteins / metabolism
Hemolysin Proteins / pharmacology
Humans
NF-kappa B / metabolism
|
Resource |
Drosophila |
11992R-1
11992R-2 |