RRC ID 81860
Author Shiino H, Furuta S, Kojima R, Kimura K, Endo T, Tamura Y.
Title Phosphatidylserine flux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA.
Journal FEBS J
Abstract Most phospholipids are synthesised in the endoplasmic reticulum and distributed to other cellular membranes. Although the vesicle transport contributes to the phospholipid distribution among the endomembrane system, exactly how phospholipids are transported to, from and between mitochondrial membranes remains unclear. To gain insights into phospholipid transport routes into mitochondria, we expressed the Escherichia coli phosphatidylserine (PS) synthase PssA in various membrane compartments with distinct membrane topologies in yeast cells lacking a sole PS synthase (Cho1). Interestingly, PssA could complement loss of Cho1 when targeted to the endoplasmic reticulum (ER), peroxisome, or lipid droplet membranes. Synthesised PS could be converted to phosphatidylethanolamine (PE) by Psd1, the mitochondrial PS decarboxylase, suggesting that phospholipids synthesised in the peroxisomes and low doses (LDs) can efficiently reach mitochondria. Furthermore, we found that PssA which has been integrated into the mitochondrial inner membrane (MIM) from the matrix side could partially complement the loss of Cho1. The PS synthesised in the MIM was also converted to PE, indicating that PS flops across the MIM to become PE. These findings expand our understanding of the intracellular phospholipid transport routes via mitochondria.
Volume 288(10)
Pages 3285-3299
Published 2021-5-1
DOI 10.1111/febs.15657
PMID 33283454
MeSH Bacterial Proteins / genetics* Bacterial Proteins / metabolism Biological Transport CDPdiacylglycerol-Serine O-Phosphatidyltransferase / deficiency CDPdiacylglycerol-Serine O-Phosphatidyltransferase / genetics* CDPdiacylglycerol-Serine O-Phosphatidyltransferase / metabolism Carboxy-Lyases / genetics Carboxy-Lyases / metabolism Endoplasmic Reticulum / metabolism Escherichia coli / genetics* Escherichia coli / metabolism Gene Expression Genetic Complementation Test Intracellular Membranes / metabolism* Kinetics Lipid Droplets / metabolism Mitochondria / metabolism Mitochondrial Proteins / genetics Mitochondrial Proteins / metabolism Peroxisomes / metabolism Phosphatidylethanolamines / biosynthesis Phosphatidylserines / metabolism* Saccharomyces cerevisiae / genetics Saccharomyces cerevisiae / metabolism Saccharomyces cerevisiae Proteins / genetics Transgenes
IF 4.392
Resource
DNA material pRS424-GPDp- PssA codon.opt. (RDB20394) pRS424-GPDp-Tom70(1-40)- PssA codon.opt. (RDB20395) pRS424-GPDp-DLD1(1-72)- PssA codon.opt. (RDB20396) pRS424-GPDp BiPN- PssA codon.opt-HDEL. (RDB20397) pRS424-GPDp- Pex3(1-60)- PssA codon.opt. (RDB20398) pRS424-GPDp- Erg6SNSRV- PssA codon.opt. (RDB20399) pRS424-GPDp- BcsIN(1-126)- PssA codon.opt. (RDB20400) pRS424-GPDp-LipIN(1-41)SNSRV.-PssA codon.opt. (RDB20401) pRS424-GPDp-Mmm1N SNSRV PssA codon.opt. (RDB20402) pRS424-GPDp-Su9- PssA codon.opt. (RDB20403) pRS424-GPDp-Tom70(1-40)- PssA(H138A) (RDB20404) pRS424-GPDp-Tom70(1-40)- PssA(H357A) (RDB20405) pRS424-GPDp-DLD1(1-72)- PssA(H138A) (RDB20406) pRS424-GPDp-DLD1(1-72)- PssA(H357A) (RDB20407) pRS424-GPDp- Erg6SNSRV- PssA(138A) (RDB20408) pRS424-GPDp- Erg6SNSRV- PssA(357A) (RDB20409) pRS424-GPDp- Bcs1N(1-126)- PssA(H138A) (RDB20410) pRS424-GPDp- Bcs1N(1-126)- PssA(H357A) (RDB20411) pRS424-GPDp-Lip1N(1-41)SNSRV.-PssA(H138A) (RDB20412) pRS424-GPDp-Lip1N(1-41)SNSRV.-PssA(H357A) (RDB20413) pRS424-GPDp-Pex3(1-60)-PssA(H138A) (RDB20414) pRS424-GPDp-Pex3(1-60)-PssA(H357A) (RDB20415)