論文 - 詳細
| RRC ID | 81868 |
|---|---|
| 著者 | Miki T, Hashimoto M, Takahashi H, Shimizu M, Nakayama S, Furuta T, Mihara H. |
| タイトル | De novo designed YK peptides forming reversible amyloid for synthetic protein condensates in mammalian cells. |
| ジャーナル | Nat Commun |
| Abstract |
In mammalian cells, protein condensates underlie diverse cell functions. Intensive synthetic biological research has been devoted to fabricating liquid droplets using de novo peptides/proteins designed from scratch in test tubes or bacterial cells. However, the development of de novo sequences for synthetic droplets forming in eukaryotes is challenging. Here, we report YK peptides, comprising 9-15 residues of alternating repeats of tyrosine and lysine, which form reversible amyloid-like fibrils accompanied by binding with poly-anion species such as ATP. By genetically tagging the YK peptide, superfolder GFPs assemble into artificial liquid-like droplets in living cells. Rational design of the YK system allows fine-tuning of the fluidity and construction of multi-component droplets. The YK system not only facilitates intracellular reconstitution of simplified models for natural protein condensates, but it also provides a toolbox for the systematic creation of droplets with different dynamics and composition for in situ evaluation. |
| 巻・号 | 15(1) |
| ページ | 8503 |
| 公開日 | 2024-10-18 |
| DOI | 10.1038/s41467-024-52708-5 |
| PII | 10.1038/s41467-024-52708-5 |
| PMID | 39424799 |
| PMC | PMC11489810 |
| MeSH | Adenosine Triphosphate / metabolism Amyloid* / chemistry Amyloid* / metabolism Animals Biomolecular Condensates / chemistry Biomolecular Condensates / metabolism Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism HeLa Cells Humans Peptides* / chemistry Peptides* / metabolism |
| IF | 12.121 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 61 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |