論文 - 詳細
| RRC ID | 81917 |
|---|---|
| 著者 | Mizutani M, Kuroda S, Oku M, Aoki W, Masuya T, Miyoshi H, Murai M. |
| タイトル | Identification of proteins involved in intracellular ubiquinone trafficking in Saccharomyces cerevisiae using artificial ubiquinone probe. |
| ジャーナル | Biochim Biophys Acta Bioenerg |
| Abstract |
Ubiquinone (UQ) is an essential player in the respiratory electron transfer system. In Saccharomyces cerevisiae strains lacking the ability to synthesize UQ6, exogenously supplied UQs can be taken up and delivered to mitochondria through an unknown mechanism, restoring the growth of UQ6-deficient yeast in non-fermentable medium. Since elucidating the mechanism responsible may markedly contribute to therapeutic strategies for patients with UQ deficiency, many attempts have been made to identify the machinery involved in UQ trafficking in the yeast model. However, definite experimental evidence of the direct interaction of UQ with a specific protein(s) has not yet been demonstrated. To gain insight into intracellular UQ trafficking via a chemistry-based strategy, we synthesized a hydrophobic UQ probe (pUQ5), which has a photoreactive diazirine group attached to a five-unit isoprenyl chain and a terminal alkyne to visualize and/or capture the labeled proteins via click chemistry. pUQ5 successfully restored the growth of UQ6-deficient S. cerevisiae (Δcoq2) on a non-fermentable carbon source, indicating that this UQ was taken up and delivered to mitochondria, and served as a UQ substrate of respiratory enzymes. Through photoaffinity labeling of the mitochondria isolated from Δcoq2 yeast cells cultured in the presence of pUQ5, we identified many labeled proteins, including voltage-dependent anion channel 1 (VDAC1) and cytochrome c oxidase subunit 3 (Cox3). The physiological relevance of UQ binding to these proteins is discussed. |
| 巻・号 | 1865(4) |
| ページ | 149147 |
| 公開日 | 2024-11-1 |
| DOI | 10.1016/j.bbabio.2024.149147 |
| PII | S0005-2728(24)00117-8 |
| PMID | 38906315 |
| MeSH | Mitochondria / metabolism Molecular Probes / metabolism Saccharomyces cerevisiae* / genetics Saccharomyces cerevisiae* / metabolism Saccharomyces cerevisiae Proteins* / genetics Saccharomyces cerevisiae Proteins* / metabolism Ubiquinone* / analogs & derivatives Ubiquinone* / metabolism |
| IF | 4.441 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 酵母 | BYP1805 |