Reference - Detail
| RRC ID | 81923 |
|---|---|
| Author | Nguyen T, Yoshikawa T, Ito Y, Kasuya H, Nakashima T, Okamoto S, Amaishi Y, Zhang H, Li Y, Matsukawa T, Inoue S, Kagoya Y. |
| Title | Protocol to measure human IL-6 secretion from CAR T cell-primed macrophage and monocyte lineage cells in vitro and in vivo using humanized mice. |
| Journal | STAR Protoc |
| Abstract |
Chimeric antigen receptor (CAR) T cell therapy often causes serious toxicities, such as cytokine release syndrome (CRS), mainly due to interleukin-6 (IL-6) secreted by monocyte lineage cells. Here, we describe a protocol to generate anti-CD19 CAR T cells and quantify human monocyte-derived IL-6 cocultured with CAR T cells and target tumor cells in vitro. We further describe a protocol to generate a humanized mouse model and evaluate CAR T cell-associated plasma IL-6 levels in vivo. For complete details on the use and execution of this protocol, please refer to Yoshikawa et al.1. |
| Volume | 5(4) |
| Pages | 103423 |
| Published | 2024-11-1 |
| DOI | 10.1016/j.xpro.2024.103423 |
| PII | S2666-1667(24)00588-4 |
| PMID | 39488831 |
| PMC | PMC11567045 |
| Resource | |
| Cord blood stem cells for research | |
| Human and Animal Cells | NALM-6(RCB1933) |